Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Department of Molecular Biosciences, The Wenner-Gren Institute, The Arrhenius Laboratories F3, Stockholm University, Stockholm, Sweden.
Nat Metab. 2022 Aug;4(8):978-994. doi: 10.1038/s42255-022-00619-4. Epub 2022 Aug 15.
Measurement of oxygen consumption is a powerful and uniquely informative experimental technique. It can help identify mitochondrial mechanisms of action following pharmacologic and genetic interventions, and characterize energy metabolism in physiology and disease. The conceptual and practical benefits of respirometry have made it a frontline technique to understand how mitochondrial function can interface with-and in some cases control-cell physiology. Nonetheless, an appreciation of the complexity and challenges involved with such measurements is required to avoid common experimental and analytical pitfalls. Here we provide a practical guide to oxygen consumption measurements covering the selection of experimental models and instrumentation, as well as recommendations for the collection, interpretation and normalization of data. These guidelines are provided with the intention of aiding experimental design and enhancing the overall reputability, transparency and reliability of oxygen consumption measurements.
氧耗测量是一种强大且独特的信息实验技术。它可以帮助识别药物和遗传干预后的线粒体作用机制，并描述生理和疾病中的能量代谢。呼吸计的概念和实际优势使其成为理解线粒体功能如何与（在某些情况下控制）细胞生理学相互作用的前沿技术。尽管如此，为了避免常见的实验和分析陷阱，仍然需要了解此类测量所涉及的复杂性和挑战。在这里，我们提供了一个实用的氧耗测量指南，涵盖了实验模型和仪器的选择，以及数据的收集、解释和归一化的建议。这些指南的目的是帮助实验设计，并提高氧耗测量的整体声誉、透明度和可靠性。
