Department of Life Sciences and Chemistry, Constructor University Bremen, Bremen, Germany.
Dokuz Eylül University, Faculty of Science, Department of Chemistry (Biochemistry Division), İzmir, Turkey.
Methods Mol Biol. 2023;2673:453-474. doi: 10.1007/978-1-0716-3239-0_30.
For the development of multi-peptide vaccine, identification of antigenic epitopes is crucial. If it is done using wet lab techniques, the identification process can be time-consuming, laborious, and cost-intensive. In silico tools, on the other hand, enable researchers to predict potential epitopes with little to no cost for further in vivo and in vitro testing. The rapid identification process using in silico tools helps in responding to health emergencies faster. Developing an efficient and high coverage vaccine is one of the ways to reduce morbidity and mortality rates of the diseases and protect the affected populations. In this chapter, we introduce the necessary tools and methodology for the identification and characterization of antigenic epitopes to design a multi-epitope vaccine using varicella-zoster virus as an example vector model.
为了开发多肽疫苗，鉴定抗原表位至关重要。如果使用湿实验室技术，则鉴定过程可能既耗时、费力又成本高昂。另一方面，计算工具使研究人员能够以几乎无需成本的方式预测潜在的表位，以便进一步进行体内和体外测试。使用计算工具进行的快速鉴定过程有助于更快地应对卫生紧急情况。开发高效、高覆盖率的疫苗是降低疾病发病率和死亡率并保护受影响人群的方法之一。在本章中，我们将介绍鉴定和表征抗原表位的必要工具和方法，并用水痘带状疱疹病毒作为示例载体模型来设计一种多表位疫苗。
