Institute of Nano Biomedicine and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China.
Biomater Sci. 2023 Jul 25;11(15):5060-5077. doi: 10.1039/d3bm00476g.
Programmable modular reaction, combined with excellent biocompatibility, has endowed biological circuits based on the nucleic acid TMSD (toehold-mediated strand displacement) reaction with great potential for bioanalysis and biomedical research. In this review, we summarize recent research on the principles of TMSD reaction and its applications in the cell environment. Based on the basic reaction units of the TD (toehold displacement) reaction, TE (toehold exchange) reaction, and CD (cooperation displacement) reaction, different reaction models could be obtained through the recombination of the basic reaction units. We highlight the successful development of the application of TMSD reaction for cell recognition, targeted therapy, molecular sensing and imaging and gene manipulations in the cell environment. Finally, we discuss the challenges and future opportunities of TMSD reaction for cellular applications.
可编程模块化反应，结合出色的生物相容性，使基于核酸 TMSD（发夹介导链位移）反应的生物电路在生物分析和生物医学研究中具有巨大的潜力。在这篇综述中，我们总结了 TMSD 反应原理及其在细胞环境中的应用的最新研究进展。基于 TD（发夹位移）反应、TE（发夹交换）反应和 CD（协同置换）反应的基本反应单元，通过基本反应单元的重组可以得到不同的反应模型。我们重点介绍了 TMSD 反应在细胞识别、靶向治疗、分子传感和成像以及基因操作在细胞环境中的应用的成功发展。最后，我们讨论了 TMSD 反应在细胞应用方面面临的挑战和未来机遇。
