Gui Jue, Azad Md Abul Kalam, Lin Wenchao, Meng Chengwen, Hu Xin, Cui Yadong, Lan Wei, He Jianhua, Kong Xiangfeng
Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutrition Physiology and Metabolic Processes, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, China.
School of Biology and Food Engineering, Fuyang Normal University, Fuyang, Anhui, China.
Front Microbiol. 2023 May 16;14:1185806. doi: 10.3389/fmicb.2023.1185806. eCollection 2023.
Chinese medicinal herbs play important roles in anti-inflammatory, antioxidant, and antibacterial activities. However, the effects of Chinese herb ultrafine powder (CHUP) on laying hens still need to be elucidated. Therefore, this study aimed to evaluate the effects of dietary CHUP supplementation on jejunal morphology, physical barrier function, and microbiota in laying hens.
A total of 576 Xinyang black-feather laying hens (300 days old) were randomly assigned into eight groups, with eight replicates per group and nine hens per replicate. The hens were fed a basal diet (control group) and a basal diet supplemented with 0.5% (LH group), 0.25% (LF group), 0.25% (TH group), 0.5% LH + 0.25% LF (LH-LF group), 0.5% LH + 0.25% TH (LH-TH group), 0.25% LF + 0.25% TH (LF-TH group), and 0.5% LH + 0.25% LF + 0.25% TH (LH-LF-TH group), respectively, for 120 days.
The results showed that dietary LH-LF and LH-LF-TH supplementation increased ( < 0.05) the jejunal villus height to crypt depth ratio of laying hens. Dietary LF-TH supplementation up-regulated jejunal expression, while LH supplementation up-regulated jejunal expression and increased the jejunal abundances of potentially beneficial bacteria related to short-chain fatty acids and bacteriocins production, such as , , , and ( < 0.05). In addition, dietary LH supplementation enriched ( < 0.05) the tetracycline biosynthesis, butirosin/neomycin biosynthesis, and D-arginine/D-ornithine metabolism, whereas steroid biosynthesis and limonene/pinene degradation were enriched ( < 0.05) in the LH-LF and LH-LF-TH groups. Moreover, Spearman's correlation analysis revealed the potential correlation between the abundance of the jejunal microbiota and jejunal morphology and the physical barrier function of laying hens.
Collectively, these findings suggest that dietary CHUP supplementation could enhance the beneficial bacteria abundance, physical barrier function, and metabolic function associated with short-chain fatty acids and bacteriocins production. Moreover, combined supplementation of dietary CHUP showed better effects than the sole CHUP supplementation.
中草药在抗炎、抗氧化和抗菌活性方面发挥着重要作用。然而，中草药超细粉（CHUP）对蛋鸡的影响仍有待阐明。因此，本研究旨在评估日粮中添加CHUP对蛋鸡空肠形态、物理屏障功能和微生物群的影响。
将576只300日龄的信阳黑羽蛋鸡随机分为8组，每组8个重复，每个重复9只鸡。分别给鸡饲喂基础日粮（对照组）和添加0.5% （LH组）、0.25% （LF组）、0.25% （TH组）、0.5% LH + 0.25% LF（LH-LF组）、0.5% LH + 0.25% TH（LH-TH组）、0.25% LF + 0.25% TH（LF-TH组）和0.5% LH + 0.25% LF + 0.25% TH（LH-LF-TH组）的基础日粮，持续120天。
结果表明，日粮中添加LH-LF和LH-LF-TH可提高（<0.05）蛋鸡空肠绒毛高度与隐窝深度之比。日粮中添加LF-TH可上调空肠 表达，而添加LH可上调空肠 表达，并增加与短链脂肪酸和细菌素产生相关的潜在有益细菌的空肠丰度，如 、 、 和 （<0.05）。此外，日粮中添加LH可富集（<0.05）四环素生物合成、丁胺卡那霉素/新霉素生物合成和D-精氨酸/D-鸟氨酸代谢，而LH-LF和LH-LF-TH组中类固醇生物合成和柠檬烯/蒎烯降解得到富集（<0.05）。此外，Spearman相关性分析揭示了蛋鸡空肠微生物群丰度与空肠形态和物理屏障功能之间的潜在相关性。
总体而言，这些发现表明，日粮中添加CHUP可提高与短链脂肪酸和细菌素产生相关的有益细菌丰度、物理屏障功能和代谢功能。此外，日粮中联合添加CHUP的效果优于单独添加CHUP。
