Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Research Center of Food Fermentation Engineering and Technology, Hubei University of Technology, Wuhan 430068, China.
Bioproducts Institute, Department of Chemical & Biological Engineering, Department of Chemistry, and Department of Wood Science, The University of British Columbia, 2360 East Mall, Vancouver BC V6T 1Z3, Canada.
Biomacromolecules. 2023 Jun 12;24(6):2541-2548. doi: 10.1021/acs.biomac.3c00053. Epub 2023 Jun 2.
The lignin-carbohydrate complexes (LCCs) typically present in the liquors produced in the pre-hydrolysis of biomass cause severe difficulties in downstream fractionation. To address this issue, a series of LCC samples were accessed from solutions obtained from the pre-hydrolysis of extractive-free pine wood meal (H-LCC) and compared with LCC obtained from the corresponding residues (B-LCC). Chromatographic and spectroscopic techniques revealed that 8.2% of the lignins were degraded at 160 °C, resulting from the breakage of β-O-4' linkages during pre-hydrolysis. Meanwhile, (reactive) hemicelluloses were mainly removed from the fibers' cell walls. Some hemicelluloses in the pre-hydrolysis liquor, such as glucomannans, were associated with degraded lignin fragments via ether and ester bonds. However, the newly formed LCCs were pH-labile and underwent rapid hydrolysis. Overall, we reveal details about LCC formation and degradation during pre-hydrolysis at given temperatures, critically important in efforts to improve biomass processing and valorization.
木质素-碳水化合物复合体（LCCs）通常存在于生物质预水解过程中产生的蒸煮液中，这给下游的分级分离带来了严重的困难。为了解决这个问题，我们从提取过的松木粉（H-LCC）预水解得到的溶液中提取了一系列 LCC 样品，并与相应残渣（B-LCC）中的 LCC 进行了比较。色谱和光谱技术表明，在 160°C 下有 8.2%的木质素发生了降解，这是由于预水解过程中β-O-4'键的断裂造成的。同时，（反应性）半纤维素主要从纤维细胞壁中被去除。预水解液中的一些半纤维素，如葡甘露聚糖，通过醚键和酯键与降解的木质素片段结合。然而，新形成的 LCC 是 pH 不稳定的，并迅速水解。总的来说，我们揭示了在给定温度下预水解过程中 LCC 的形成和降解的细节，这对于改善生物质处理和增值具有重要意义。
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