Université libre de Bruxelles (ULB), École polytechnique de Bruxelles, 3BIO-BioMatter, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050 Brussels, Belgium; Department of Process Engineering and Technology of Polymer and Carbon Materials, Faculty of Chemistry, Wroclaw University of Science and Technology, Norwida 4/6, 50-373 Wroclaw, Poland.
Université libre de Bruxelles (ULB), École polytechnique de Bruxelles, 3BIO-BioMatter, Avenue F.D. Roosevelt, 50 - CP 165/61, 1050 Brussels, Belgium; Université Paris Saclay, Polytech Paris Saclay, Rue Louis de Broglie, 91400 Orsay, France.
Int J Biol Macromol. 2023 Jul 31;244:125201. doi: 10.1016/j.ijbiomac.2023.125201. Epub 2023 Jun 2.
In this study, we developed a well-printable biomaterial ink for 3D printing of shape-maintaining hydrogel scaffolds. The hydrogel base comprised tyramine-modified hyaluronic acid (HA-Tyr) and gelatin methacrylate (GelMA) and was dually cross-linked. Using the Box-Behnken design, we explored how varying the ink composition affected fiber formation and shape preservation. By adjusting the polymer ratios, we produced a stable hydrogel with varying responses, from a viscous liquid to a thick gel, and optimized 3D scaffolds that were structurally stable both during and after printing, offering precision and flexibility. Our ink exhibited shear-thinning behavior and high swelling capacity, as well as ECM-like characteristics and biocompatibility, making it an ideal candidate for soft tissues matrices with storage modulus of around 300 Pa. Animal trials and CAM assays confirmed its biocompatibility and integration with host tissue.
在这项研究中，我们开发了一种可精确保留形状的水凝胶支架 3D 打印用生物相容性墨水。该水凝胶基底由经过 tyramine 修饰的透明质酸（HA-Tyr）和甲基丙烯酰化明胶（GelMA）组成，采用双重交联方式。通过 Box-Behnken 设计，我们探索了改变墨水组成如何影响纤维形成和形状保留。通过调整聚合物比例，我们生产出了一种具有不同响应的稳定水凝胶，从粘性液体到浓稠凝胶，优化了 3D 支架，使其在打印过程中和之后都具有结构稳定性，提供了精确性和灵活性。我们的墨水表现出剪切稀化行为和高溶胀能力，以及类似细胞外基质的特性和生物相容性，使其成为具有约 300 Pa 存储模量的软组织基质的理想候选物。动物试验和 CAM 测定证实了其生物相容性和与宿主组织的整合性。
