[大鼠肝脏赖氨酰 - tRNA合成酶的物理化学性质]
[Physico-chemical properties of lysyl-tRNA-synthetase from the rat liver].
作者信息
Verkhogliad I N, Vinogradova R P, Kucherenko N E
出版信息
Ukr Biokhim Zh (1978). 1985 Jul-Aug;57(4):19-23.
Two lysyl-tRNA-synthetase forms are obtained from the rat liver. Their molecular masses are determined by electrophoresis and gel-filtration on Sephadex G-150: form I-122, form II-64 kDalton. Gel-electrophoresis in the presence of 0.1% SDS indicates that form I of lysyl-tRNA-synthetase consists of two subunits with a molecular mass of 64 kDalton each, i. e. it is a dimer. Optimal conditions and kinetic parameters (Km and Vmax) of aminoacylation for the both enzyme forms are similar. Amino acid composition, fluorescence parameters and thermal inactivation conditions are determined.
从大鼠肝脏中获得了两种赖氨酰 - tRNA合成酶形式。通过在Sephadex G - 150上进行电泳和凝胶过滤测定它们的分子量：形式I为122kDa，形式II为64kDa。在0.1% SDS存在下的凝胶电泳表明，赖氨酰 - tRNA合成酶的形式I由两个分子量均为64kDa的亚基组成，即它是一种二聚体。两种酶形式的氨酰化最佳条件和动力学参数（Km和Vmax）相似。测定了氨基酸组成、荧光参数和热失活条件。
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