Department of Cariology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India.
Department of Anatomy, Biomedical Research Unit and Laboratory Animal Centre (BRULAC), Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, 600077, India.
Mol Biol Rep. 2024 Jan 7;51(1):89. doi: 10.1007/s11033-023-09146-1.
Kappaphycus alvarezii, a marine red algae species, has gained significant attention in recent years due to its versatile bioactive compounds. Among these, κ-carrageenan (CR), a sulfated polysaccharide, exhibits remarkable antimicrobial properties. This study emphasizes the synergism attained by functionalizing zinc oxide nanoparticles (ZnO NPs) with CR, thereby enhancing its antimicrobial efficacy and target specificity against dental pathogens.
In this study, we synthesized ZnO-CR NPs and characterized them using SEM, FTIR, and XRD techniques to authenticate their composition and structural attributes. Moreover, our investigation revealed that ZnO-CR NPs possess better free radical scavenging capabilities, as evidenced by their effective activity in the DPPH and ABTS assay.
The antimicrobial properties of ZnO-CR NPs were systematically assessed using a zone of inhibition assay against dental pathogens of S. aureus, S. mutans, E. faecalis, and C. albicans, demonstrating their substantial inhibitory effects at a minimal concentration of 50 μg/mL. We elucidated the interaction between CR and the receptors of dental pathogens to further understand their mechanism of action. The ZnO-CR NPs demonstrated a dose-dependent anticancer effect at concentrations of 5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL on KB cells, a type of Human Oral Epidermal Carcinoma. The mechanism by which ZnO-CA NPs induced apoptosis in KB cells was determined by observing an increase in the expression of the BCL-2, BAX, and P53 genes.
Our findings unveil the promising potential of ZnO-CR NPs as a candidate with significant utility in dental applications. The demonstrated biocompatibility, potent antioxidant and antiapoptotic activity, along with impressive antimicrobial efficacy position these NPs as a valuable resource in the ongoing fight against dental pathogens and oral cancer.
卡拉胶是一种海洋红藻，由于其具有多种生物活性化合物，近年来受到了广泛关注。其中，κ-卡拉胶（CR）是一种硫酸多糖，具有显著的抗菌性能。本研究强调了通过功能化氧化锌纳米粒子（ZnO NPs）与 CR 的协同作用，从而提高其对口腔病原体的抗菌功效和靶向特异性。
在这项研究中，我们合成了 ZnO-CR NPs，并通过 SEM、FTIR 和 XRD 技术对其进行了表征，以验证其组成和结构属性。此外，我们的研究表明，ZnO-CR NPs 具有更好的自由基清除能力，这一点在 DPPH 和 ABTS 测定中得到了有效证明。
我们通过抑菌圈试验系统地评估了 ZnO-CR NPs 对口腔病原体金黄色葡萄球菌、变形链球菌、粪肠球菌和白色念珠菌的抗菌性能，结果表明，它们在 50μg/mL 的最小浓度下就具有显著的抑制作用。我们阐明了 CR 与口腔病原体受体之间的相互作用，以进一步了解其作用机制。在浓度为 5μg/mL、25μg/mL、50μg/mL 和 100μg/mL 的条件下，ZnO-CR NPs 对 KB 细胞（一种人口腔表皮癌细胞）表现出剂量依赖性的抗癌作用。通过观察 BCL-2、BAX 和 P53 基因表达的增加，确定了 ZnO-CA NPs 诱导 KB 细胞凋亡的机制。
我们的研究结果揭示了 ZnO-CR NPs 在口腔应用中具有很大潜力的候选物。这些 NPs 具有良好的生物相容性、强大的抗氧化和抗凋亡活性，以及显著的抗菌功效，使其成为对抗口腔病原体和口腔癌的一种有价值的资源。
Zotero 插件