Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology, Kattankulathur 603 203, Chennai, Tamil Nadu, India.
Department of Biotechnology, College of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur 603 203, Chennai, Tamil Nadu, India.
Comp Biochem Physiol C Toxicol Pharmacol. 2022 Dec;262:109463. doi: 10.1016/j.cbpc.2022.109463. Epub 2022 Sep 7.
Copper sulfate (CuSO) as industrial effluent is intentionally or unintentionally released into water bodies and accumulates in the fish. Because of its numerous applications, CuSO can be hazardous to non-target creatures, producing direct alterations in fish habitats. Acacetin is a flavonoid present in all vascular plants that are extensively dispersed in plant pigments and responsible for many natural hues. However, the impact of acacetin on mitigating the toxic effect of CuSO in the in-vivo conditions is not known. The toxicity of acacetin was determined by measuring the survival, deformities and heart rate after treatment with various concentrations to larvae. The protective effect of acacetin was also observed in CuSO exposed zebrafish larvae by reducing malformation, mortality rate and oxidative stress. Meanwhile, the acacetin-protected larvae from CuSO effects through the molecular mechanism by suppressing pro-inflammatory genes (COX-2, TNF-α and IL-1) and upregulating antioxidant genes (GPx, GST and GR). Overall, our findings suggest that acacetin can act as a protective barrier against CuSO-induced inflammation in an in-vivo zebrafish larval model.
硫酸铜（CuSO）作为工业废水被故意或无意地排放到水体中，并在鱼类中积累。由于其广泛的应用，CuSO 可能对非目标生物有害，直接改变鱼类的栖息地。芹黄素是一种存在于所有维管植物中的类黄酮，广泛分布在植物色素中，是许多天然颜色的成因。然而，芹黄素在减轻体内 CuSO 毒性方面的影响尚不清楚。通过测量不同浓度处理后幼虫的存活率、畸形率和心率来确定芹黄素的毒性。在暴露于 CuSO 的斑马鱼幼虫中也观察到了芹黄素的保护作用，通过减少畸形率、死亡率和氧化应激来减轻 CuSO 的毒性。同时，芹黄素通过抑制促炎基因（COX-2、TNF-α 和 IL-1）和上调抗氧化基因（GPx、GST 和 GR）来保护幼虫免受 CuSO 影响，从分子机制上保护幼虫。总的来说，我们的研究结果表明，芹黄素可以作为一种保护屏障，防止体内斑马鱼幼虫模型中 CuSO 诱导的炎症。
