Zeng Sujuan, Wu Yuejun, Wang Lijing, Huang Yuhang, Huang Wenyan, Li Ziling, Gao Weijian, Jiang Siqing, Ge Lihong, Zhang Jian
Department of Pedodontics, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine, Guangzhou, 510182, China.
Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
Heliyon. 2023 May 22;9(6):e16545. doi: 10.1016/j.heliyon.2023.e16545. eCollection 2023 Jun.
The purpose of this study was to explore the feasibility of using optical coherence tomography (OCT) for real-time and quantitative monitoring of enamel development in gene-edited enamel defect mice. NF-κB activator 1, known as Act1, is associated with many inflammatory diseases. The antisense oligonucleotide of Act1 was inserted after the CD68 gene promoter, which would cover the start region of the Act1 gene and inhibit its transcription. Anti-Act1 mice, gene-edited mice, were successfully constructed and demonstrated amelogenesis imperfecta by scanning electron microscope (SEM) and energy dispersive X-ray (EDX) spectroscopy. Wild-type (WT) mice were used as the control group in this study. WT mice and anti-Act1 mice at 3 weeks old were examined by OCT every week and killed at eight weeks old. Their mandibular bones were dissected and examined by OCT, micro-computed tomography (micro-CT), and SEM. OCT images showed that the outer layer of enamel of anti-Act1 mice was obviously thinner than that of WT mice but no difference in total thickness. When assessing enamel thickness, there was a significant normal linear correlation between these methods. OCT could scan the imperfect developed enamel noninvasively and quickly, providing images of the enamel layers of mouse incisors.
本研究的目的是探讨使用光学相干断层扫描（OCT）对基因编辑的釉质发育不全小鼠的釉质发育进行实时定量监测的可行性。核因子κB激活剂1（Act1）与许多炎症性疾病有关。Act1的反义寡核苷酸插入到CD68基因启动子之后，这将覆盖Act1基因的起始区域并抑制其转录。成功构建了抗Act1小鼠（基因编辑小鼠），并通过扫描电子显微镜（SEM）和能量色散X射线（EDX）光谱证实其釉质形成不全。野生型（WT）小鼠用作本研究的对照组。对3周龄的WT小鼠和抗Act1小鼠每周进行一次OCT检查，并在8周龄时处死。解剖它们的下颌骨，并用OCT、微型计算机断层扫描（micro-CT）和SEM进行检查。OCT图像显示，抗Act1小鼠的釉质外层明显比WT小鼠薄，但总厚度无差异。在评估釉质厚度时，这些方法之间存在显著的正常线性相关性。OCT可以无创、快速地扫描发育不全的釉质，提供小鼠切牙釉质层的图像。
