Zeng Sujuan, Huang Yuhang, Huang Wenyan, Pathak Janak L, He Yanbing, Gao Weijian, Huang Jing, Zhang Yiqing, Zhang Jian, Dong Huixian
Department of Pediatric Dentistry, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou Key Laboratory of Basic and Applied Research of Regenerative Medicine, Guangzhou 510182, China.
Department of Biomedical Engineering, School of Basic Medical Sciences, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Guangzhou Medical University, Guangzhou 511436, China.
Diagnostics (Basel). 2021 Nov 4;11(11):2046. doi: 10.3390/diagnostics11112046.
The aim of the present study was to explore the feasibility of real-time monitoring and quantitative guiding the repair of enamel white spot lesions (WSLs) with resin infiltration by optical coherence tomography (OCT). Seven New Zealand rabbits were treated with 37% phosphoric acid etchant for 15 min to establish the model of enamel demineralization chalk spots of upper incisors, which were repaired by Icon resin infiltrant. OCT, stereo microscope (SM) imaging, scanning electron microscope (SEM) imaging and hematoxylin eosin (HE) staining were used to image each operation step. The changes of WSLs of enamel before and in the process of restoration with resin infiltrant showed specific performance in OCT images, which were consistent with the corresponding results of stereomicroscope and SEM. OCT can non-invasively and accurately image the whole process of repairing enamel demineralization layer with resin infiltration real-time, which can effectively guide the clinical use of resin infiltrant to repair enamel WSLs and be used as an imaging tool to evaluate the process and effect of restoration with resin infiltrant at the same time.
本研究的目的是探讨通过光学相干断层扫描（OCT）对树脂浸润修复牙釉质白斑病变（WSLs）进行实时监测和定量指导的可行性。选用7只新西兰兔，用37%磷酸酸蚀剂处理15分钟，建立上颌切牙牙釉质脱矿白垩斑模型，并用Icon树脂浸润剂进行修复。采用OCT、体视显微镜（SM）成像、扫描电子显微镜（SEM）成像和苏木精-伊红（HE）染色对各操作步骤进行成像。牙釉质WSLs在树脂浸润修复前及修复过程中的变化在OCT图像中表现出特定特征，与体视显微镜和SEM的相应结果一致。OCT能够实时、无创且准确地对树脂浸润修复牙釉质脱矿层的全过程进行成像，可有效指导树脂浸润剂在临床修复牙釉质WSLs中的应用，同时作为一种成像工具评估树脂浸润修复的过程和效果。
