Kaspersen Håkon, Urdahl Anne Margrete, Franklin-Alming Fiona Valerie, Ilag Hanna Karin, Hetland Marit A K, Bernhoff Eva, Löhr Iren H, Sunde Marianne
Section for Food Safety and Animal Health, Norwegian Veterinary Institute, Ås, Norway.
Section for Microbiology, Norwegian Veterinary Institute, Ås, Norway.
Front Microbiol. 2023 May 18;14:1193274. doi: 10.3389/fmicb.2023.1193274. eCollection 2023.
is an important opportunistic pathogen widely studied in relation to human infection and colonization. However, there is a lack of knowledge regarding other niches that may inhabit. isolated from healthy broiler and turkey flocks in Norway in 2018 have previously been described with regard to population structure, sequence types (STs), and the presence of virulence- and antimicrobial resistance (AMR) genes. In the present study we aimed to evaluate the dynamics of the population in poultry over time, with regards to AMR and virulence, and with a special focus on persistence of STs. A total of 391 flocks sampled in 2020 were included in the present study, of which 271 were from broiler flocks and 120 from turkey flocks. Similar to findings from 2018, the occurrence of was significantly higher based on culturing in turkey flocks (62.5%) compared to broiler flocks (24.0%). Major STs in 2020 included ST5827 ( = 7), ST37 ( = 7), ST370 ( = 7), ST17 ( = 5), and ST4710 ( = 5). Several STs persisted over time in both host species, including ST35, ST37, ST590, and ST17. This persistence may be due to local re-circulation or reintroduction from parent flocks. Of these five major STs, only ST590 carried AMR genes, indicating that the persistence was not associated with the presence of AMR genes. An ST4710 strain with a hypervirulence-encoding plasmid (p4710; , ) was recovered from turkeys in 2018. The same strain was present in turkeys in 2020, but the plasmid had lost the salmochelin locus. This loss may be attributed to reductive evolution due to the presence of several siderophores within the same isolates. In this study we also characterized a clinical ST4710 isolate from a turkey with airsacculitis. The isolate was closely related to two intestinal ST4710 isolates from healthy turkeys in 2018. These three isolates were sampled within the same location and time frame in 2018, and all carried the full p4710 virulence plasmid. These findings highlight the transmission- and infectious potential of ST4710 in turkeys.
是一种重要的机会性病原体，在人类感染和定植方面得到了广泛研究。然而，对于它可能栖息的其他生态位，我们了解不足。2018年从挪威健康肉鸡和火鸡群中分离出的[病原体名称未给出]，此前已在种群结构、序列类型（STs）以及毒力和抗菌药物耐药性（AMR）基因的存在方面进行了描述。在本研究中，我们旨在评估家禽中[病原体名称未给出]种群随时间的动态变化，涉及AMR和毒力，并特别关注STs的持续性。本研究共纳入了2020年采样的391个鸡群，其中271个来自肉鸡群，120个来自火鸡群。与2018年的研究结果相似，基于培养结果，火鸡群中[病原体名称未给出]的发生率（62.5%）显著高于肉鸡群（24.0%）。2020年的主要STs包括ST5827（=7）、ST37（=7）、ST370（=7）、ST17（=5）和ST4710（=5）。几种STs在两种宿主物种中都随时间持续存在，包括ST35、ST37、ST590和ST17。这种持续性可能是由于本地再循环或来自亲本鸡群的重新引入。在这五个主要STs中，只有ST590携带AMR基因，这表明持续性与AMR基因的存在无关。2018年从火鸡中分离出一株携带高毒力编码质粒（p4710；[具体信息未给出]）的ST4710菌株。2020年在火鸡中也发现了同一菌株，但该质粒失去了沙门菌素基因座。这种丢失可能归因于同一分离株中存在多种铁载体导致的还原性进化。在本研究中，我们还对一株来自患有气囊炎的火鸡的临床ST4710分离株进行了特征分析。该分离株与2018年来自健康火鸡的两株肠道ST4710分离株密切相关。这三株分离株在2018年的同一地点和时间范围内采样，并且都携带完整的p4710毒力质粒。这些发现突出了ST4710在火鸡中的传播和感染潜力。
