Mestrado em Ciências Farmacêuticas, CCS, Universidade do Vale do Itajaí, Itajaí, SC, Brazil.
J Ethnopharmacol. 2010 Feb 3;127(2):543-50. doi: 10.1016/j.jep.2009.09.061. Epub 2009 Oct 4.
The aerial parts of Baccharis dracunculifolia D.C., popularly known as "alecrim do campo", are used in folk medicine as anti-inflammatory. The aim of the present study was to evaluate the anti-inflammatory and antinociceptive activities of the crude hydroalcoholic extract obtained from leaves of Baccharis dracunculifolia (BdE), which have not been reported.
BdE was analyzed by HPLC and in vivo evaluated (doses ranging from 50 to 400mg/kg, p.o.) by using the acetic acid-induced abdominal constrictions, paw oedema induced by carrageenan or histamine, overt nociception models using capsaicin, glutamate or phorbol myristate acetate (PMA), formalin-induced nociception and mechanical hypernociception induced by carrageenan or complete Freund adjuvant (CFA). As positive controls it was used paracetamol in both acetic acid and formalin tests; dipyrone in capsaicin, glutamate and PMA-induced nociception; indomethacin in CFA and carrageenan-induced hypernociception models. In addition, the in vitro effects of BdE on COX-2 activity and on the activation of NF-kappaB were also evaluated.
BdE (50-400mg/kg, p.o.) significantly diminished the abdominal constrictions induced by acetic acid, glutamate and CFA. Furthermore, BdE also inhibited the nociceptive responses in both phases of formalin-induced nociception. BdE, administered orally, also produced a long-lasting anti-hypernociceptive effect in the acute model of inflammatory pain induced by carrageenan. It was also observed the inhibition of COX-2 activity by BdE.
In summary, the data reported in this work confirmed the traditional anti-inflammatory indications of Baccharis dracunculifolia leaves and provided biological evidences that Baccharis dracunculifolia, like Brazilian green propolis, possess antinociceptive and anti-inflammatory activities.
Baccharis dracunculifolia D.C. 的地上部分，俗称“alecrim do campo”，在民间医学中被用作抗炎药。本研究旨在评估 Baccharis dracunculifolia（BdE）叶的粗水醇提取物的抗炎和镇痛活性，目前尚未有报道。
通过 HPLC 对 BdE 进行分析，并通过醋酸诱导的腹部收缩、角叉菜胶或组胺诱导的爪肿胀、辣椒素、谷氨酸或佛波醇肉豆蔻酸乙酯（PMA）引起的显性疼痛模型、福尔马林诱导的疼痛和角叉菜胶或完全弗氏佐剂（CFA）引起的机械性痛觉过敏来进行体内评估（剂量范围为 50 至 400mg/kg，po）。作为阳性对照，在醋酸和福尔马林试验中使用对乙酰氨基酚；在辣椒素、谷氨酸和 PMA 引起的疼痛中使用双氯芬酸；在 CFA 和角叉菜胶诱导的痛觉过敏模型中使用吲哚美辛。此外，还评估了 BdE 对 COX-2 活性和 NF-kappaB 激活的体外作用。
BdE（50-400mg/kg，po）显著减少了醋酸、谷氨酸和 CFA 诱导的腹部收缩。此外，BdE 还抑制了福尔马林诱导的疼痛的两个阶段的疼痛反应。BdE 经口给药还可在角叉菜胶诱导的急性炎症性疼痛模型中产生持久的抗痛觉过敏作用。还观察到 BdE 抑制 COX-2 活性。
综上所述，本工作中报告的数据证实了 Baccharis dracunculifolia 叶的传统抗炎适应症，并提供了生物学证据，表明 Baccharis dracunculifolia 与巴西绿蜂胶一样具有镇痛和抗炎活性。
