戊二醛-豌豆蛋白接枝多糖基质作为共价固定化剂的作用。
Glutaraldehyde-pea protein grafted polysaccharide matrices for functioning as covalent immobilizers.
机构信息
Department of Chemistry of Natural and Microbial Products, National Research Centre, El-Behooth St., Dokki, Giza, Egypt.
Centre of Scientific Excellence-Group of Advanced Materials and Nanotechnology, National Research Centre, El-Behooth St., Dokki, Giza, Egypt.
出版信息
Sci Rep. 2023 Jun 5;13(1):9105. doi: 10.1038/s41598-023-36045-z.
Three polysaccharide matrices (κ-Carrageenan (Carr), gellan gum, and agar) were grafted via glutaraldehyde (GA) and pea protein (PP). The grafted matrices covalently immobilized β-D-galactosidase (β-GL). Nonetheless, grafted Carr acquired the topmost amount of immobilized β-GL (iβ-GL). Thus, its grafting process was honed via Box-Behnken design and was further characterized via FTIR, EDX, and SEM. The optimal GA-PP-Carr grafting comprised processing Carr beads with 10% PP dispersion of pH 1 and 25% GA solution. The optimal GA-PP-Carr beads acquired 11.44 Ug iβ-GL with 45.49% immobilization efficiency. Both free and GA-PP-Carr iβ-GLs manifested their topmost activity at the selfsame temperature and pH. Nonetheless, the β-GL K and V values were reduced following immobilization. The GA-PP-Carr iβ-GL manifested good operational stability. Moreover, its storage stability was incremented where 91.74% activity was offered after 35 storage days. The GA-PP-Carr iβ-GL was utilized to degrade lactose in whey permeate with 81.90% lactose degradation efficiency.
三种多糖基质（κ-卡拉胶（Carr）、结冷胶和琼脂）通过戊二醛（GA）和豌豆蛋白（PP）接枝。接枝的基质通过β-D-半乳糖苷酶（β-GL）共价固定化。然而，接枝的 Carr 获得了最多量的固定化β-GL（iβ-GL）。因此，通过 Box-Behnken 设计对其接枝过程进行了优化，并通过 FTIR、EDX 和 SEM 进行了进一步表征。最佳的 GA-PP-Carr 接枝包括用 10% PP 分散体 pH 1 和 25% GA 溶液处理 Carr 珠。最佳的 GA-PP-Carr 珠获得了 11.44 Ug iβ-GL，固定化效率为 45.49%。游离的和 GA-PP-Carr iβ-GLs 在相同的温度和 pH 下表现出最高的活性。然而，β-GL 的 K 和 V 值在固定化后降低。GA-PP-Carr iβ-GL 表现出良好的操作稳定性。此外，其储存稳定性得到提高，在 35 天的储存后提供了 91.74%的活性。GA-PP-Carr iβ-GL 用于降解乳清渗透物中的乳糖，乳糖降解效率为 81.90%。
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