Enzymology Laboratory, Food Science and Technology Institute, Federal University of Rio Grande do Sul, 9500 Bento Gonçalves Ave, P.O. Box 15090, ZC 91501-970 Porto Alegre, RS, Brazil.
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires & CONICET-UBA, Centro de Investigaciones en Hidratos de Carbono (CIHIDECAR), Intendente Güiraldes 2160, Ciudad Universitaria, C1428EGA Buenos Aires, Argentina.
Carbohydr Polym. 2022 Sep 1;291:119483. doi: 10.1016/j.carbpol.2022.119483. Epub 2022 Apr 13.
In this work, we studied the development of a biocomposite formulated with alginate and gelatin, crosslinked with genipin for application as support for β-galactosidase immobilization. Also, the biocomposites with the immobilized enzyme were characterized by thermal analyses and SAXS (size, density, and interconnectivity of alginate rods) for a detailed analysis of the microstructure, as well as the thermal and operational stabilities of the enzyme. The structural modifications of the biocomposite determined by SAXS demonstrate that the addition of both genipin and enzyme produced a significant reduction in size and density of the Ca(II)-alginate rods. Immobilized β-galactosidase could be stored for 175 days under refrigeration maintaining 80% of its initial activity. Moreover, 90% of its relative activity was kept after 11 reuses in a batch process of lactose hydrolysis. Thus, the biocomposite proved to be effective as support for enzyme immobilization.
在这项工作中，我们研究了一种由海藻酸钠和明胶组成的生物复合材料的发展，该复合材料用京尼平交联，用作β-半乳糖苷酶固定化的载体。此外，通过热分析和小角 X 射线散射（海藻酸钠棒的大小、密度和连通性）对具有固定化酶的生物复合材料进行了表征，以对其微观结构进行详细分析，以及酶的热稳定性和操作稳定性。小角 X 射线散射确定的生物复合材料的结构修饰表明，京尼平和酶的添加都导致 Ca(II)-海藻酸钠棒的尺寸和密度显著降低。固定化β-半乳糖苷酶在冷藏条件下可储存 175 天，保持初始活性的 80%。此外，在乳糖水解的批处理过程中重复使用 11 次后，其相对活性仍保持 90%。因此，该生物复合材料被证明是一种有效的酶固定化载体。
