Studying the Bioactive Components and Phytochemicals of the Methanol Extract of Rhanterium epapposum Oliv.

Rhanterium epapposum Oliv. (locally known as Al-Arfaj) belongs to the family Asteraceae. This study was designed to discover the bioactive components and phytochemicals of the methanol extract of the aerial parts of Rhanterium epapposum, using Agilent Gas Chromatography-Mass Spectrometry (GC-MS), while the mass spectra of the compounds found in the extract matched with the National Institute of Standards and Technology (NIST08 L). GC-MS analysis of the methanol extract of Rhanterium epapposum aerial parts showed presence of sixteen compounds. The major compounds among these were 9,12,15-octadecatrienoic acid, (Z, Z, Z)- (9.89), n-hexadecenoic acid (8.44), 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one (6.60), benzene propanoic acid, β-amino-4-methoxy- (6.12), 1.4-isopropyl-1,6-dimethyl-1,2,3,4,4 a,7,8,8a-octahedron-1-naphthalenol (6.00), 1-dodecanol, 3,7,11-trimethyl- (5.64), and 9,12-octadecadienoic acid (Z, Z)- (4.84), whereas the minor compounds were 9-Octadecenoic acid, (2-phenyl-1,3-dioxolan-4-yl)methyl ester, trans- (3.63), Butanoic acid (2.93), Stigmasterol (2.92), 2-Naphthalenemethanol (2.66), (2,6,6-Trimethylcyclohex-1-phenylmethanesulfonyl)benzene (2.45), 2-(Ethylenedioxy) ethylamine, N-methyl-N-[4-(1-pyrrolidinyl)-2-butynyl]- (2.00), 1-Heptatriacotanol (1.69), Ocimene (1.59), and β-Sitosterol (1.25). Furthermore, the study was extended to determine the phytochemicals in the methanol extract of Rhanterium epapposum, which indicated the positive presence of saponins, flavonoids, and phenolic compounds. Moreover, quantitative analysis revealed the presence of high content of flavonoids, total phenolic, and tannins. This study outcome suggests a podium of using Rhanterium epapposum aerial parts as a herbal remedy for various diseases especially cancers, hypertension, and diabetes.