Department of Veterinary Medicine, College of Agricultural and Veterinary Medicine, Qassim University, Buraydah, Saudi Arabia.
Department of Pharmacology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
Appl Biochem Biotechnol. 2024 May;196(5):2414-2424. doi: 10.1007/s12010-023-04574-y. Epub 2023 Jun 6.
Rhanterium epapposum Oliv. (locally known as Al-Arfaj) belongs to the family Asteraceae. This study was designed to discover the bioactive components and phytochemicals of the methanol extract of the aerial parts of Rhanterium epapposum, using Agilent Gas Chromatography-Mass Spectrometry (GC-MS), while the mass spectra of the compounds found in the extract matched with the National Institute of Standards and Technology (NIST08 L). GC-MS analysis of the methanol extract of Rhanterium epapposum aerial parts showed presence of sixteen compounds. The major compounds among these were 9,12,15-octadecatrienoic acid, (Z, Z, Z)- (9.89), n-hexadecenoic acid (8.44), 7-hydroxy-6-methoxy-2H-1-benzopyran-2-one (6.60), benzene propanoic acid, β-amino-4-methoxy- (6.12), 1.4-isopropyl-1,6-dimethyl-1,2,3,4,4 a,7,8,8a-octahedron-1-naphthalenol (6.00), 1-dodecanol, 3,7,11-trimethyl- (5.64), and 9,12-octadecadienoic acid (Z, Z)- (4.84), whereas the minor compounds were 9-Octadecenoic acid, (2-phenyl-1,3-dioxolan-4-yl)methyl ester, trans- (3.63), Butanoic acid (2.93), Stigmasterol (2.92), 2-Naphthalenemethanol (2.66), (2,6,6-Trimethylcyclohex-1-phenylmethanesulfonyl)benzene (2.45), 2-(Ethylenedioxy) ethylamine, N-methyl-N-[4-(1-pyrrolidinyl)-2-butynyl]- (2.00), 1-Heptatriacotanol (1.69), Ocimene (1.59), and β-Sitosterol (1.25). Furthermore, the study was extended to determine the phytochemicals in the methanol extract of Rhanterium epapposum, which indicated the positive presence of saponins, flavonoids, and phenolic compounds. Moreover, quantitative analysis revealed the presence of high content of flavonoids, total phenolic, and tannins. This study outcome suggests a podium of using Rhanterium epapposum aerial parts as a herbal remedy for various diseases especially cancers, hypertension, and diabetes.
瑞特姆角胡麻（俗称阿尔法杰）属于菊科。本研究旨在通过安捷伦气相色谱-质谱联用仪（GC-MS）发现瑞特姆角胡麻地上部分甲醇提取物中的生物活性成分和植物化学物质，而提取物中发现的化合物的质谱与美国国家标准与技术研究所（NIST08L）相匹配。GC-MS 分析瑞特姆角胡麻地上部分甲醇提取物表明存在十六种化合物。其中主要化合物为 9,12,15-十八碳三烯酸，（Z,Z,Z）-（9.89），正十六烯酸（8.44），7-羟基-6-甲氧基-2H-1-苯并吡喃-2-酮（6.60），苯丙酸，β-氨基-4-甲氧基-（6.12），1.4-异丙基-1,6-二甲基-1,2,3,4,4a,7,8,8a-八氢-1-萘酚（6.00），1-十二烷醇，3,7,11-三甲基-（5.64）和 9,12-十八碳二烯酸（Z,Z）-（4.84），而少量化合物为 9-十八碳烯酸，（2-苯基-1,3-二恶烷-4-基）甲基酯，反式（3.63），丁酸（2.93），豆甾醇（2.92），2-萘甲醇（2.66），（2,6,6-三甲基环己-1-苯甲磺酸基）苯（2.45），2-（乙二氧基）乙基胺，N-甲基-N-[4-（1-吡咯烷基）-2-丁炔基]-（2.00），1-十七烷三酰醇（1.69），奥克米酮（1.59）和β-谷甾醇（1.25）。此外，该研究还扩展到确定瑞特姆角胡麻甲醇提取物中的植物化学物质，这表明存在皂角苷、类黄酮和酚类化合物。此外，定量分析显示出高含量的类黄酮、总酚和单宁。本研究结果表明，瑞特姆角胡麻地上部分可作为治疗各种疾病的草药，特别是癌症、高血压和糖尿病。
