State Institution "D. F. Chebotarev Institute of Gerontology NAMS Ukraine, Kiev, Ukraine.
Educational and Scientific Institute of High Technologies, Taras Shevchenko National University of Kyiv, Kiev, Ukraine.
J Mol Neurosci. 2023 Jun;73(6):448-455. doi: 10.1007/s12031-023-02130-1. Epub 2023 Jun 6.
Telomere attrition is attributed to Alzheimer's disease (AD), major depressive disorder, stress levels, physical inactivity, short sleep duration, and reduced educational abilities. In this article, we tried to assess the association between the telomere length in peripheral blood leukocytes and level of cognitive impairment and its dependence on age and sex. Healthy subjects and patients with amnestic mild cognitive impairment (aMCI) and different AD stages were recruited in the study. All patients were assessed by the same standard diagnostic procedure, including neurological examination-Mini-Mental State Examination (MMSE). Blood samples from 66 subjects (18 men and 48 women, mean age 71.2 ± 0.56 years) were collected for DNA extraction from peripheral mononuclear cells (PBMC). Relative telomere length (RTL) was measured by monochrome multiplex polymerase chain reaction. The data obtained in the study indicate that RTL in PBMCs has a statistically significant association with MMSE score (p < 0.02). Moreover, the sex-specific difference was observed for the association between telomere length and various parameters of MMSE. Also, it has been found that a decrease in RTL by one unit is associated with an increase in the odds to get AD at a ratio of 2.54 (95% CI, 1.25 to 5.17). The results obtained in this research are in coherence with other studies that telomere length may be a valuable biomarker of cognitive decline. However, the potential need for longitudinal studies of telomere length, in order to estimate the influence of hereditary and environmental factors, remains.
端粒损耗归因于阿尔茨海默病（AD）、重度抑郁症、压力水平、身体活动不足、睡眠持续时间短和受教育程度降低。在本文中，我们试图评估外周血白细胞端粒长度与认知障碍程度的相关性及其对年龄和性别的依赖性。研究中招募了健康受试者和有遗忘型轻度认知障碍（aMCI）和不同 AD 阶段的患者。所有患者均通过相同的标准诊断程序进行评估，包括神经系统检查-简易精神状态检查（MMSE）。从 66 名受试者（18 名男性和 48 名女性，平均年龄 71.2 ± 0.56 岁）的血液样本中提取外周单核细胞（PBMC）的 DNA。通过单色多重聚合酶链反应测量相对端粒长度（RTL）。研究中获得的数据表明，PBMC 中的 RTL 与 MMSE 评分有统计学显著相关性（p < 0.02）。此外，还观察到端粒长度与 MMSE 各种参数之间的关联存在性别特异性差异。此外，还发现 RTL 减少一个单位与 AD 的发病几率增加 2.54 倍（95%CI，1.25 至 5.17）相关。该研究结果与其他研究一致，即端粒长度可能是认知能力下降的有价值的生物标志物。然而，为了估计遗传和环境因素的影响，仍然需要对端粒长度进行纵向研究。
