Department of Agricultural Chemistry, Faculty of Agriculture, Minia University, El-Minia, Egypt.
Environ Sci Pollut Res Int. 2023 Jul;30(32):79067-79081. doi: 10.1007/s11356-023-27827-z. Epub 2023 Jun 7.
This study investigates blackberry juice's effects on glucose metabolism, oxidative stress, inflammation, and endoplasmic reticulum stress (ER) in streptozotocin (STZ)-induced diabetic rats. Fifty Wistar rats were distributed to five groups randomly of ten rats each: Normal control, diabetic control, 9 mL/kg body weight (b.wt) blackberry juice only, blackberry juice plus diabetes, and 500 mg/kg b.wt metformin plus diabetes. A single intraperitoneal injection of 50 mg/kg b.wt STZ induced diabetes in the rats. This animal study continued for 56 days after the confirmation of diabetes. The levels of liver function and renal function, as well as insulin, glucose-6-phosphatase, glucokinase, and malondialdehyde (MDA) levels, and the activities of catalase (CAT) and superoxide dismutase (SOD), were determined. Additionally, interleukin-6 (IL-6) levels, tumor necrosis factor-alpha (TNF-α), and activated transcription factor 4 (ATF4) expressions were examined in the liver homogenate of rats. Furthermore, the liver tissues were utilized for histopathological examination. The results showed that blackberry juice prevented drastic loss of body weight and reduced food consumption in diabetic rats. Additionally, the levels of blood glucose, total protein, aspartate aminotransferase (AST), albumin, alanine aminotransferase (ALT), uric acid, creatinine, and urea improved after the administration of blackberry juice in diabetic rats. Blackberry juice significantly increased glucose metabolism and antioxidant status while reducing ER stress and inflammation in diabetic rats. Moreover, blackberry juice improved glucose metabolism by increasing insulin levels and improving the dysregulated activities of glucose-metabolizing enzymes. The microstructure of liver tissues in diabetic rats was also improved with blackberry juice treatment. Therefore, blackberry juice has the potential to alleviate diabetes in rats and could be considered as a functional food for people with diabetes.
本研究旨在探讨黑莓汁对链脲佐菌素（STZ）诱导的糖尿病大鼠葡萄糖代谢、氧化应激、炎症和内质网应激（ER）的影响。将 50 只 Wistar 大鼠随机分为 5 组，每组 10 只：正常对照组、糖尿病对照组、9mL/kg 体重黑莓汁组、黑莓汁加糖尿病组和 500mg/kg 体重二甲双胍加糖尿病组。大鼠一次性腹腔注射 50mg/kg 体重 STZ 诱导糖尿病。在糖尿病确诊后，这项动物研究持续了 56 天。测定肝功能、肾功能、胰岛素、葡萄糖-6-磷酸酶、葡萄糖激酶和丙二醛（MDA）水平，以及过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的活性。此外，还检测了大鼠肝匀浆中白细胞介素-6（IL-6）水平、肿瘤坏死因子-α（TNF-α）和激活转录因子 4（ATF4）的表达。进一步利用肝组织进行组织病理学检查。结果表明，黑莓汁可防止糖尿病大鼠体重明显减轻和摄食量减少。此外，黑莓汁可改善糖尿病大鼠的血糖、总蛋白、天门冬氨酸氨基转移酶（AST）、白蛋白、丙氨酸氨基转移酶（ALT）、尿酸、肌酐和尿素水平。黑莓汁可显著改善糖尿病大鼠的葡萄糖代谢和抗氧化状态，同时减轻 ER 应激和炎症。此外，黑莓汁通过增加胰岛素水平和改善葡萄糖代谢酶的失调活性来改善葡萄糖代谢。黑莓汁还可改善糖尿病大鼠肝组织的微观结构。因此，黑莓汁具有缓解大鼠糖尿病的潜力，可作为糖尿病患者的功能性食品。
