Kitamura Tomiichiro, Kobayashi Satoru, Okada Masukichi
Institute of Biological Sciences University of Tsukuba Tsukuba, Ibaraki 305, Japan.
Dev Growth Differ. 1993 Feb;35(1):67-73. doi: 10.1111/j.1440-169X.1993.00067.x.
In Drosophila, it has been suggested that the third intron of the P element is spliced out only in germ-line cells. However, we found that the splicing took place in some somatic tissues during embryogenesis, as well as in pole cells, progenitor cells of the germ line. To detect the splicing activity in vivo, we designed an assay system to visualize the cells where the third intron was spliced out by the histochemical staining for β-galactosidase activity. In this paper, we describe the somatic tissues where the third intron is spliced out during embryogenesis, with reference to the stages when the splicing occurs. Based on these findings we propose a modification of the previous documentation that the complete processing of transcripts from P element takes place only in germ-line cells.
在果蝇中，有人提出P因子的第三个内含子仅在生殖系细胞中被剪接去除。然而，我们发现剪接过程在胚胎发育期间的一些体细胞组织以及生殖系的祖细胞极细胞中也会发生。为了检测体内的剪接活性，我们设计了一个检测系统，通过对β-半乳糖苷酶活性进行组织化学染色来可视化第三个内含子被剪接去除的细胞。在本文中，我们描述了胚胎发育期间第三个内含子被剪接去除的体细胞组织，并参考了剪接发生的阶段。基于这些发现，我们建议对之前的文献记录进行修正，即P因子转录本的完全加工仅在生殖系细胞中进行。
