Chain A C, Zollman S, Tseng J C, Laski F A
Department of Biology, University of California, Los Angeles 90024.
Mol Cell Biol. 1991 Mar;11(3):1538-46. doi: 10.1128/mcb.11.3.1538-1546.1991.
P element transposition in Drosophila melanogaster is limited to the germ line because the third intron (the ORF2-ORF3 intron) of the P element transcript is spliced only in germ line cells. We describe a systematic search for P element sequences that are required to regulate the splicing of the ORF2-ORF3 intron. We have identified three adjacent mutations that abolish the germ line specificity and allow splicing of this intron in all tissues. These mutations define a 20-base regulatory region located in the exon, 12 to 31 bases from the 5' splice site. Our data show that this cis-acting regulatory sequence is required to inhibit the splicing of the ORF2-ORF3 intron in somatic cells.
在黑腹果蝇中，P 因子转座仅限于生殖系，因为 P 因子转录本的第三个内含子（即 ORF2 - ORF3 内含子）仅在生殖系细胞中进行剪接。我们描述了一项对调控 ORF2 - ORF3 内含子剪接所需的 P 因子序列进行的系统搜索。我们鉴定出三个相邻的突变，这些突变消除了生殖系特异性，并允许该内含子在所有组织中进行剪接。这些突变定义了一个位于外显子中的 20 个碱基的调控区域，距离 5' 剪接位点 12 至 31 个碱基。我们的数据表明，这个顺式作用调控序列对于抑制体细胞中 ORF2 - ORF3 内含子的剪接是必需的。
