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Analysis of the cis-acting requirements for germ-line-specific splicing of the P-element ORF2-ORF3 intron.

作者信息

Laski F A, Rubin G M

机构信息

Department of Biochemistry, University of California, Berkeley 94720.

出版信息

Genes Dev. 1989 May;3(5):720-8. doi: 10.1101/gad.3.5.720.

DOI:10.1101/gad.3.5.720
PMID:2545526
Abstract

P-element transposition is limited to the germ line because the element's third intron is only spliced in germ line cells. We show that a 240-bp fragment containing this 190-bp intron can confer germ line specificity when placed in the context of another gene. We find that the cis-acting regulatory sequences required for germ line regulation map near to, but not at, the 5' or 3' splice junctions.

摘要

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