Edgell M H, Perler F, Blin N, Hutchinson C A, Stafford D W
Natl Cancer Inst Monogr. 1978 May(48):11-8.
A fine structure map of the ribosomal gene region of the sea urchin Lytechinus variegatus has been generated. The ribosomal DNA density satellite has been analyzed with restriction enzymes and by hybridization to urchin 18S and 26S ribosomal RNA (rRNA). The L. variegatus rRNA repeats show heterogeneity on the nontranscribed spacer. Minor bands containing transcribed spacer sequences are observed. One interpretation of these bands would require both transcribed spacer heterogeneity and defective ribosomal genes retained among the rRNA gene repeats.
已经生成了多斑丽海胆核糖体基因区域的精细结构图。利用限制性内切酶并通过与海胆18S和26S核糖体RNA(rRNA)杂交,对核糖体DNA密度卫星进行了分析。多斑丽海胆rRNA重复序列在非转录间隔区表现出异质性。观察到含有转录间隔区序列的小条带。对这些条带的一种解释需要转录间隔区的异质性以及rRNA基因重复序列中保留的有缺陷的核糖体基因。