海胆18S - 26S rRNA基因间隔区的核苷酸序列。
Nucleotide sequence of the 18S-26S rRNA intergene region of the sea urchin.
作者信息
Hindenach B R, Stafford D W
出版信息
Nucleic Acids Res. 1984 Feb 10;12(3):1737-47. doi: 10.1093/nar/12.3.1737.
Abstract
The DNA sequence which spans the internal transcribed spacers of a cloned ribosomal transcription unit from the sea urchin, Lytechinus variegatus, has been determined. The region extends from the conserved Eco RI site near the 3' end of the 18S rDNA to a Bam HI site in the 26S rDNA and includes 232 nucleotides coding for 18S rRNA, 367 nucleotides of internal transcribed spacer, 159 nucleotides coding for 5.8S rRNA, 338 nucleotides of internal transcribed spacer, and 505 nucleotides coding for 26S rRNA. The rRNA coding regions were identified by direct analysis of 3'-labeled 18S and 5.8S rRNA and 5'-labeled 5.8S rRNA, and by sequence homology of the 26S rDNA with yeast and vertebrate 26/28S rRNAs. The internal transcribed spacers are GC-rich, similar to those of vertebrates. The 5.8S and 5' 26S rDNA sequences support a proposed model for a structural domain of the yeast large subunit ribosomal RNA (Veldman et al. [1981] Nucleic Acids Res. 9, 6935-6952).
摘要
已确定来自海胆（Lytechinus variegatus）的一个克隆核糖体转录单位的内部转录间隔区的DNA序列。该区域从18S rDNA 3'端附近的保守Eco RI位点延伸至26S rDNA中的一个Bam HI位点，包括编码18S rRNA的232个核苷酸、内部转录间隔区的367个核苷酸、编码5.8S rRNA的159个核苷酸、内部转录间隔区的338个核苷酸以及编码26S rRNA的505个核苷酸。通过对3'-标记的18S和5.8S rRNA以及5'-标记的5.8S rRNA进行直接分析，以及通过26S rDNA与酵母和脊椎动物26/28S rRNA的序列同源性来鉴定rRNA编码区。内部转录间隔区富含GC，类似于脊椎动物的间隔区。5.8S和5' 26S rDNA序列支持酵母大亚基核糖体RNA结构域的一个提议模型（Veldman等人，[1981]《核酸研究》9，6935 - 6952）。
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