多棘海胆（Lytechinus variegatus）核糖体RNA基因转录起始区域

The region of transcriptional initiation in Lytechinus variegatus rRNA genes.

作者信息

Bieber D, Blin N, Stafford D W

出版信息

Biochim Biophys Acta. 1981 Oct 27;655(3):366-73. doi: 10.1016/0005-2787(81)90047-2.

Abstract

A sea urchin ribosomal DNA 1.9 kilobase BamHI fragment adjacent to the 5' end of the 18 S gene has been mapped with the restriction enzymes, XhoI, EcoRi, SmaI and HinfI. A 270 basepair fragment which most likely contains the 5' end of the presumed primary transcript of rRNA was identified by hybridization of [32P]DNA fragments to total nuclear RNA separated on methylmercury hydroxide gels and bound to diazobenzyloxymethyl paper. Under these denaturing conditions the size of Lytechinus variegatus precursor rRNA was determined to be 7.2 kilobases (33 S).

摘要

与18 S基因5′端相邻的海胆核糖体DNA 1.9千碱基对BamHI片段已用限制性内切酶XhoI、EcoRI、SmaI和HinfI进行了图谱分析。通过将[32P]DNA片段与在氢氧化甲基汞凝胶上分离并结合到重氮苄氧基甲基纸上的总核RNA杂交，鉴定出一个最有可能包含rRNA假定初级转录本5′端的270碱基对片段。在这些变性条件下，确定多色海胆前体rRNA的大小为7.2千碱基对（33 S）。