Yamazaki Kimie, Suzuki Rika, Hojo Eriko, Kondo Shunzo, Kato Yoshihiro, Kamioka Ken, Hoshi Motonori, Sawada Hitoshi
Mitsubishi-Kasei Institute of Life Sciences, Machida, Tokyo 194, Japan.
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Yokohama 227, Japan.
Dev Growth Differ. 1994 Apr;36(2):149-154. doi: 10.1111/j.1440-169X.1994.00149.x.
Effects of twelve protease inhibitors on hatching of mouse embryos were investigated. Mouse hatching was strongly or moderately inhibited by trypsin inhibitors including p-toluenesulfonyl-Lys-CH Cl (TLCK) and chicken ovomucoid, while inhibitors for chymotrypsin and elastase showed weak or no inhibition. These results indicate the participation of a trypsin-like protease in the hatching of mouse embryos as a hatching enzyme., Since TLCK is the strongest and an irreversible inhibitor for the enzyme, timing of the participation of the hatching enzyme in the hatching process was examined by pulse treatment of embryos with TLCK before and during the zona shedding. The results indicated that a trypsin-like hatching enzyme functions before, but not during, the zona shedding of embryos, especially during a 15 h period immediately before the beginning of the shedding.
研究了十二种蛋白酶抑制剂对小鼠胚胎孵化的影响。包括对甲苯磺酰-L-赖氨酸氯甲基酮（TLCK）和鸡卵类粘蛋白在内的胰蛋白酶抑制剂对小鼠孵化有强烈或中等程度的抑制作用，而糜蛋白酶和弹性蛋白酶的抑制剂则表现出较弱的抑制作用或无抑制作用。这些结果表明，一种类胰蛋白酶作为孵化酶参与了小鼠胚胎的孵化过程。由于TLCK是该酶最强的不可逆抑制剂，通过在透明带脱落之前和期间用TLCK对胚胎进行脉冲处理，研究了孵化酶参与孵化过程的时间。结果表明，类胰蛋白酶孵化酶在胚胎透明带脱落之前起作用，但在脱落过程中不起作用，尤其是在脱落开始前的15小时内。
