Elliott A Y, Dombrovskis S, Fraley E E
Natl Cancer Inst Monogr. 1978 Dec(49):199-201.
The IFA assay has been used to detect antigen--antibody reactions between sera from patients with TCC and TCC cell lines established in our laboratory. A positive immunofluorescence reaction, consisting of perinuclear staining, was seen in 86% of the sera from TCC patients exposed to 253J cells. A positive reaction was detection in only 8% of the sera from healthy donors, in 19% of that from patients with nonmalignant urinary tract disease, and in 31% of the sera from patients with urinary tract tumors other than TCC when exposed to 253J cells. No positive reaction was detected when these same sera were exposed to either WI-38 or normal human kidney cells. The positive fluorescence could be removed from TCC sera by absorption with TCC surgical specimens but not by absorption with nontumor surgical specimens.
间接荧光抗体试验（IFA）已用于检测移行细胞癌（TCC）患者血清与我们实验室建立的TCC细胞系之间的抗原 - 抗体反应。在暴露于253J细胞的TCC患者的86%血清中观察到阳性免疫荧光反应，其表现为核周染色。当暴露于253J细胞时，仅8%健康供者血清、19%非恶性泌尿系统疾病患者血清以及31%除TCC外的泌尿系统肿瘤患者血清检测到阳性反应。当这些相同血清暴露于WI - 38细胞或正常人肾细胞时，未检测到阳性反应。TCC血清中的阳性荧光可通过与TCC手术标本吸附而去除，但不能通过与非肿瘤手术标本吸附而去除。
