Fujian Provincial Key Laboratory of Innovative Drug Target Research and State Key Laboratory of Cellular Stress Biology, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China.
BE/Phase I Clinical Center, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen 361000, China.
Biomater Sci. 2023 Jul 25;11(15):5078-5094. doi: 10.1039/d3bm00529a.
Non-viral polymeric vectors with good biocompatibility have been recently explored as delivery systems for clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) nucleases. In this review, based on current limitations and critical barriers, we summarize the advantages of stimulus-responsive polymeric delivery vectors (, pH, redox, or enzymes) towards controllable CRISPR/Cas9 genome editing system delivery as well as the advances in using stimulus-responsive CRISPR/Cas9 polymeric carriers towards cancer treatment. Last but not least, the key challenges and promising development strategies of stimulus-responsive polymeric vector designs for CRISPR/Cas9 systems will also be discussed.
最近，人们探索了具有良好生物相容性的非病毒聚合物载体作为成簇规律间隔短回文重复序列 (CRISPR)-相关 (Cas) 核酸酶的递药系统。在这篇综述中，我们根据目前的局限性和关键障碍，总结了刺激响应性聚合物递药载体（pH、氧化还原或酶）在可控 CRISPR/Cas9 基因组编辑系统递送上的优势，以及利用刺激响应性 CRISPR/Cas9 聚合物载体在癌症治疗方面的进展。最后但同样重要的是，我们还将讨论 CRISPR/Cas9 系统中刺激响应性聚合物载体设计的关键挑战和有前途的发展策略。
