Qi Yu-Tong, Zhang Miao, Meng Shuo, Ren Jun-Guo, Liu Jian-Xun
Beijing Key Laboratory of Pharmacology of Chinese Materia Medica, Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences Beijing 100091, China.
Zhongguo Zhong Yao Za Zhi. 2023 Apr;48(7):1858-1865. doi: 10.19540/j.cnki.cjcmm.20221125.301.
To optimize the extraction process of Chuanxiong Rhizoma-Gastrodiae Rhizoma herb pair by network pharmacology combined with analytic hierarchy process(AHP)-entropy weight method and multi-index orthogonal test. The potential active components and targets of Chuanxiong Rhizoma-Gastrodiae Rhizoma were screened by network pharmacology and molecular docking, and the process evaluation indexes were determined with reference to the Chinese Pharmacopoeia(2020 edition). The core components of Chuanxiong Rhizoma-Gastrodiae Rhizoma were determined as gastrodin, parishin B, parishin C, parishin E, ferulic acid, and 3-butylphthalide. With the extraction volume of each indicator and yield of dry extract as comprehensive evaluation indicators, the extraction conditions were optimized by the AHP-entropy weight method and orthogonal test as the ethanol volume of 50%, the solid-liquid ratio of 1∶8(g·mL~(-1)), extraction for three times, and 1.5 h each time. Through network pharmacology and molecular docking, the process evaluation index was determined, and the optimized process was stable and reproducible for the extraction of Chuanxiong Rhizoma-Gastrodiae Rhizoma herb pair, which could provide reference for in-depth research.
采用网络药理学结合层次分析法（AHP）-熵权法及多指标正交试验优化川芎-天麻药对的提取工艺。通过网络药理学和分子对接筛选川芎-天麻的潜在活性成分和靶点，并参照《中国药典》（2020年版）确定工艺评价指标。确定川芎-天麻的核心成分为天麻素、羌活醇B、羌活醇C、羌活醇E、阿魏酸和3-丁基苯酞。以各指标提取量和干膏得率为综合评价指标，采用层次分析法-熵权法结合正交试验优化提取条件为乙醇体积分数50%、固液比1∶8（g·mL⁻¹）、提取3次、每次1.5 h。通过网络药理学和分子对接确定了工艺评价指标，优化后的工艺对川芎-天麻药对的提取稳定、可重复，可为深入研究提供参考。
