Li Ping, Xu Jin-Guo, Ji De, Mao Chun-Qin, Zhang Ke-Wei, Song Yan, Lu Tu-Lin
Nanjing University of Chinese Medicine Nanjing 210023,China.
Shanghai Huayu Pharmaceutical Co.,Ltd. Shanghai 200002,China.
Zhongguo Zhong Yao Za Zhi. 2019 Oct;44(20):4460-4466. doi: 10.19540/j.cnki.cjcmm.20190630.310.
Totally 21 batches of Gastrodiae Rhizoma pieces from various habitats with different appearance characteristics were analyzed. Five active components( gastrodin,4-hydroxybenzyl alcohol,parishin B,parishin C,and parishin A) were determined by UPLC. Polysaccharide content was determined by phenol sulfuric acid method. And the content of alcohol extracts was determined. The correlation between appearance characteristics and active components,polysaccharide content and amount of alcohol extracts of Gastrodiae Rhizoma pieces was statistically analyzed by SPSS 19. 0 software. And the five active components in the 21 Gastrodiae Rhizoma pieces were clustered by using the Ward' s method in SPSS 19. 0 software. The study found that inconspicuous keratinous Gastrodiae Rhizoma had a low content of each component,and the degree of keratin was positively correlated with the content of each component and the extract,with no significant correlation with the polysaccharide content. In the cluster analysis,the 21 batches were divided into three groups,except the second group whose gastrodin content was significantly higher than the special conditions of other groups. According to the content of the five active components,19 batches of Gastrodiae Rhizoma pieces were classified into two groups with obvious keratin and no obvious keratin. In terms of color,with the deepening of brown color,the content of each component showed a downward trend. The grading of decoction pieces of Gastrodiae Rhizoma shall give full consideration to the color and texture. And the combination of chemical composition and appearance characteristics is an indicator to evaluate the product specification standards.
对来自不同产地、具有不同外观特征的21批天麻饮片进行了分析。采用超高效液相色谱法测定了天麻素、4-羟基苯甲醇、巴利森苷B、巴利森苷C和巴利森苷A这5种活性成分。采用苯酚硫酸法测定多糖含量，并测定了醇提取物的含量。运用SPSS 19.0软件对天麻饮片的外观特征与活性成分、多糖含量及醇提取物量之间的相关性进行了统计分析。并运用SPSS 19.0软件中的Ward法对21批天麻饮片中的5种活性成分进行聚类分析。研究发现，角质不明显的天麻各成分含量较低，角质化程度与各成分及提取物含量呈正相关，与多糖含量无显著相关性。聚类分析中，21批天麻饮片分为3组，除第2组天麻素含量显著高于其他组的特殊情况外。根据5种活性成分的含量，19批天麻饮片分为角质明显和角质不明显两组。在颜色方面，随着褐色加深，各成分含量呈下降趋势。天麻饮片的等级划分应充分考虑颜色和质地。化学成分与外观特征相结合是评价产品规格标准的指标。
