de Souza Gabriela Leite, Freitas Gabrielle Alves Nunes, Ribeiro Maria Tereza Hordones, Lemus Nelly Xiomara Alvarado, Soares Carlos José, Moura Camilla Christian Gomes
Department of Endodontics, School of Dentistry, Federal University of Uberlândia, Uberlândia, Brazil.
School of Dentistry, Federal University of Uberlândia, Uberlândia, Brazil.
Restor Dent Endod. 2023 May 5;48(2):e21. doi: 10.5395/rde.2023.48.e21. eCollection 2023 May.
This study evaluated the effects of Biodentine (BD), Bio-C Repair (BCR), and mineral trioxide aggregate (MTA) plug on the fracture resistance of simulated immature teeth with replacement root resorption (RRR) and -induced osteoclastogenesis.
Sixty bovine incisors simulating immature teeth and RRR were divided into 5 groups: BD and BCR groups, with samples completely filled with the respective materials; MTA group, which utilized a 3-mm apical MTA plug; RRR group, which received no root canal filling; and normal periodontal ligament (PL) group, which had no RRR and no root canal filling. All the teeth underwent cycling loading, and compression strength testing was performed using a universal testing machine. RAW 264.7 macrophages were treated with 1:16 extracts of BD, BCR, and MTA containing receptor activator of nuclear factor-kappa B ligand (RANKL) for 5 days. RANKL-induced osteoclast differentiation was assessed by staining with tartrate-resistant acid phosphatase. The fracture load and osteoclast number were analyzed using 1-way ANOVA and Tukey's test (α = 0.05).
No significant difference in fracture resistance was observed among the groups ( 0.05). All materials similarly inhibited osteoclastogenesis ( 0.05), except for BCR, which led to a lower percentage of osteoclasts than did MTA ( 0.0001).
The treatment options for non-vital immature teeth with RRR did not strengthen the teeth and promoted a similar resistance to fractures in all cases. BD, MTA, and BCR showed inhibitory effects on osteoclast differentiation, with BCR yielding improved results compared to the other materials.
本研究评估了生物陶瓷（BD）、生物C修复材料（BCR）和三氧化矿物凝聚体（MTA）充填物对模拟有替代性牙根吸收（RRR）和诱导破骨细胞生成的未成熟牙齿抗折性的影响。
将60颗模拟未成熟牙齿和RRR的牛切牙分为5组：BD组和BCR组，样本分别完全用相应材料充填；MTA组，使用3mm根尖MTA充填物；RRR组，不进行根管充填；正常牙周膜（PL）组，无RRR且无根管充填。所有牙齿均进行循环加载，并使用万能试验机进行抗压强度测试。用含核因子κB受体活化因子配体（RANKL）的BD、BCR和MTA的1:16提取物处理RAW 264.7巨噬细胞5天。通过抗酒石酸酸性磷酸酶染色评估RANKL诱导的破骨细胞分化。采用单因素方差分析和Tukey检验（α = 0.05）分析抗折负荷和破骨细胞数量。
各组间抗折性无显著差异（P>0.05）。除BCR外，所有材料均同样抑制破骨细胞生成（P<0.05），BCR导致的破骨细胞百分比低于MTA（P<0.0001）。
对于有RRR的无活力未成熟牙齿，治疗方案并未增强牙齿强度，且在所有情况下均促进了相似的抗折性。BD、MTA和BCR对破骨细胞分化均有抑制作用，与其他材料相比，BCR产生了更好的效果。
