Xu Xuanming, Li Shun, Luan Xingwei, Xuan Chao, Zhao Peng, Zhou Tingting, Tian Qingwu, Pan Deng
Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.
Front Chem. 2023 May 22;11:1191075. doi: 10.3389/fchem.2023.1191075. eCollection 2023.
Imatinib (IMB), an anticancer drug, is extensively used for chemotherapy to improve the quality of life of cancer patients. The aim of therapeutic drug monitoring (TDM) is to guide and evaluate the medicinal therapy, and then optimize the clinical effect of individual dosing regimens. In this work, a highly sensitive and selective electrochemical sensor based on glassy carbon electrode (GCE) modified with acetylene black (AB) and a Cu (II) metal organic framework (CuMOF) was developed to measure the concentration of IMB. CuMOF with preferable adsorbability and AB with excellent electrical conductivity functioned cooperatively to enhance the analytical determination of IMB. The modified electrodes were characterized using X-rays diffraction (XRD), X-ray photoelectron spectroscopy (XPS), fourier transform infrared (FT-IR), ultraviolet and visible spectrophotometry (UV-vis), electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), brunauer‒emmett‒teller (BET) and barrett‒joyner‒halenda (BJH) techniques. Analytical parameters such as the ratio of CuMOF to AB, dropping volumes, pH, scanning rate and accumulation time were investigated through cyclic voltammetry (CV). Under optimal conditions, the sensor exhibited an excellent electrocatalytic response for IMB detection, and two linear detection ranges were obatined of 2.5 nM-1.0 μM and 1.0-6.0 μM with a detection limit (DL) of 1.7 nM (S/N = 3). Finally, the good electroanalytical ability of CuMOF-AB/GCE sensor facilitated the successful determination of IMB in human serum samples. Due to its acceptable selectivity, repeatability and long-term stability, this sensor shows promising application prospects in the detection of IMB in clinical samples.
伊马替尼（IMB）是一种抗癌药物，广泛用于化疗以提高癌症患者的生活质量。治疗药物监测（TDM）的目的是指导和评估药物治疗，进而优化个体化给药方案的临床效果。在本研究中，开发了一种基于乙炔黑（AB）和铜（II）金属有机框架（CuMOF）修饰的玻碳电极（GCE）的高灵敏且选择性的电化学传感器，用于测定IMB的浓度。具有良好吸附性的CuMOF和具有优异导电性的AB协同作用，增强了对IMB的分析测定。采用X射线衍射（XRD）、X射线光电子能谱（XPS）、傅里叶变换红外光谱（FT-IR）、紫外可见分光光度法（UV-vis）、电化学阻抗谱（EIS）、扫描电子显微镜（SEM）、能量色散X射线光谱（EDS）、布鲁诺尔-埃米特-泰勒（BET）和巴雷特-乔伊纳-哈伦达（BJH）技术对修饰电极进行了表征。通过循环伏安法（CV）研究了CuMOF与AB的比例、滴加体积、pH值、扫描速率和富集时间等分析参数。在最佳条件下，该传感器对IMB检测表现出优异的电催化响应，获得了2.5 nM - 1.0 μM和1.0 - 6.0 μM两个线性检测范围，检测限（DL）为1.7 nM（S/N = 3）。最后，CuMOF-AB/GCE传感器良好的电分析能力有助于成功测定人血清样品中的IMB。由于其具有可接受的选择性、重复性和长期稳定性，该传感器在临床样品中IMB的检测方面显示出广阔的应用前景。
