乳酸链球菌素作为一种强大的羊毛硫抗生素对结肠癌细胞的抗黏附作用。
The Anti-Adhesion Effect of Nisin as a Robust Lantibiotic on the Colorectal Cancer Cells.
作者信息
Soleimanifar Hesam, Mahmoodzadeh Hosseini Hamideh, Samavarchi Tehrani Sadra, Mirhosseini Seyed Ali
机构信息
Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Adv Biomed Res. 2023 Apr 28;12:113. doi: 10.4103/abr.abr_267_21. eCollection 2023.
BACKGROUND
Bacteriocins are a type of antimicrobial peptide that are produced by probiotics. They have been studied as possible therapeutic drugs and have been used to suppress bacterial development in foods. Nisin is a potent bacteriocin having the anti-microbial and anti-cancer characteristics produced by . The aim of the present paper is to evaluate the influence of Nisin on cell adhesion and its two related genes, and , in the colorectal cancer cell line.
MATERIALS AND METHODS
For this purpose, HT-29 cells were treated with various concentrations of Nisin and the cell cytotoxicity, cell adhesion, and gene expression were evaluated using the MTT assay, cell adhesion assay, and real-time PCR.
RESULTS
Our findings showed that 32 to 1024 μg/ml of Nisin resulted in a significant reduction in cell viability ( < 0.05). Furthermore, 128 and 256 μg/ml of Nisin significantly reduced the cell adhesion, and -2 and -9 gene expressions ( < 0.05).
CONCLUSION
Our findings suggested that Nisin could prevent metastasis and cancer progression.
背景
细菌素是一类由益生菌产生的抗菌肽。它们已作为潜在的治疗药物进行研究，并已用于抑制食品中的细菌生长。乳链菌肽是一种由[具体来源未提及]产生的具有抗菌和抗癌特性的强效细菌素。本文旨在评估乳链菌肽对结肠癌细胞系中细胞黏附及其两个相关基因[基因名称未提及]和[基因名称未提及]的影响。
材料与方法
为此，用不同浓度的乳链菌肽处理HT - 29细胞，并使用MTT法、细胞黏附试验和实时PCR评估细胞毒性、细胞黏附及基因表达。
结果
我们的研究结果表明，32至1024μg/ml的乳链菌肽导致细胞活力显著降低（P < 0.05）。此外，128和256μg/ml的乳链菌肽显著降低细胞黏附以及[基因名称未提及]-2和[基因名称未提及]-9基因表达（P < 0.05）。
结论
我们的研究结果表明，乳链菌肽可预防转移和癌症进展。
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