Zheng Yanfen, Li Zhiguo, Liu Chuyi, Fan Xiaotong, Luo Zheng, Li Zibiao, Wu Yun-Long
Xiamen Key Laboratory of Stomatological Disease Diagnosis and Treatment, Stomatological Hospital of Xiamen Medical College, Xiamen, Fujian, China.
China and Fujian College Engineering Research Center for Dental Biomaterials, Xiamen, China.
Front Bioeng Biotechnol. 2023 May 23;11:1194398. doi: 10.3389/fbioe.2023.1194398. eCollection 2023.
Diabetic oral mucosa ulcers face challenges of hypoxia, hyperglycemia and high oxidative stress, which result in delayed healing process. Oxygen is regarded as an important substance in cell proliferation, differentiation and migration, which is beneficial to ulcer recovery. This study developed a multi-functional GOx-CAT nanogel (GCN) system for the treatment of diabetic oral mucosa ulcers. The catalytic activity, ROS scavenge and oxygen supply ability of GCN was validated. The therapeutic effect of GCN was verified in the diabetic gingival ulcer model. The results showed that the nanoscale GCN was capable of significantly eliminating intracellular ROS, increasing intracellular oxygen concentration and accelerating cell migration of human gingival fibroblasts, which could promote diabetic oral gingival ulcer healing by alleviating inflammation and promoting angiogenesis. This multifunctional GCN with ROS depletion, continuous oxygen supply and good biocompatibility, which might provide a novel therapeutic strategy for effective treatment of diabetic oral mucosa ulcers.
糖尿病性口腔黏膜溃疡面临缺氧、高血糖和高氧化应激等挑战，这导致愈合过程延迟。氧气被认为是细胞增殖、分化和迁移中的重要物质，有利于溃疡恢复。本研究开发了一种用于治疗糖尿病性口腔黏膜溃疡的多功能葡萄糖氧化酶-过氧化氢酶纳米凝胶（GCN）系统。验证了GCN的催化活性、活性氧清除能力和氧气供应能力。在糖尿病牙龈溃疡模型中验证了GCN的治疗效果。结果表明，纳米级GCN能够显著消除细胞内活性氧，增加细胞内氧浓度并加速人牙龈成纤维细胞的迁移，这可以通过减轻炎症和促进血管生成来促进糖尿病性口腔牙龈溃疡的愈合。这种具有活性氧消耗、持续氧气供应和良好生物相容性的多功能GCN，可能为有效治疗糖尿病性口腔黏膜溃疡提供一种新的治疗策略。
