Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Bioresour Technol. 2023 Sep;384:129280. doi: 10.1016/j.biortech.2023.129280. Epub 2023 Jun 7.
Microbial biomanufacturing is a promising approach to produce high-value compounds with low-carbon footprint and significant economic benefits. Among twelve "Top Value-Added Chemicals from Biomass", itaconic acid (IA) stands out as a versatile platform chemical with numerous applications. IA is naturally produced by Aspergillus and Ustilago species through a cascade enzymatic reaction between aconitase (EC 4.2.1.3) and cis-aconitic acid decarboxylase (EC 4.1.1.6). Recently, non-native hosts such as Escherichia coli, Corynebacterium glutamicum, Saccharomyces cerevisiae, and Yarrowia lipolytica have been genetically engineered to produce IA through the introduction of key enzymes. This review provides an up-to-date summary of the progress made in IA bioproduction, from native to engineered hosts, covers in vivo and in vitro approaches, and highlights the prospects of combination tactics. Current challenges and recent endeavors are also addressed to envision comprehensive strategies for renewable IA production in the future towards sustainable development goals (SDGs).
微生物制造是一种很有前途的方法，可以生产具有低碳足迹和显著经济效益的高价值化合物。在“十二种来自生物质的高附加值化学品”中，衣康酸（IA）作为一种多功能平台化学品脱颖而出，具有众多应用。IA 是由 Aspergillus 和 Ustilago 属通过 aconitase（EC 4.2.1.3）和 cis-aconitic 酸脱羧酶（EC 4.1.1.6）之间的级联酶反应天然产生的。最近，通过引入关键酶，非天然宿主如 Escherichia coli、Corynebacterium glutamicum、Saccharomyces cerevisiae 和 Yarrowia lipolytica 已被基因工程化用于生产 IA。本综述提供了从天然宿主到工程宿主的 IA 生物生产进展的最新总结，涵盖了体内和体外方法，并强调了联合策略的前景。还讨论了当前的挑战和最近的努力，以展望未来实现可持续发展目标（SDGs）的可再生 IA 生产的综合策略。
