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利用废食用油生产衣康酸的工程技术。

Engineering to Produce Itaconic Acid From Waste Cooking Oil.

作者信息

Rong Lanxin, Miao Lin, Wang Shuhui, Wang Yaping, Liu Shiqi, Lu Zhihui, Zhao Baixiang, Zhang Cuiying, Xiao Dongguang, Pushpanathan Krithi, Wong Adison, Yu Aiqun

机构信息

State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.

Food, Chemical and Biotechnology Cluster, Singapore Institute of Technology, Dover, Singapore.

出版信息

Front Bioeng Biotechnol. 2022 Apr 25;10:888869. doi: 10.3389/fbioe.2022.888869. eCollection 2022.

DOI:10.3389/fbioe.2022.888869
PMID:35547171
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9083544/
Abstract

Itaconic acid (IA) is a high-value organic acid with a plethora of industrial applications. In this study, we seek to develop a microbial cell factory that could utilize waste cooking oil (WCO) as raw material for circular and cost-effective production of the abovementioned biochemical. Specifically, we expressed cis-aconitic acid decarboxylase (CAD) gene from in either the cytosol or peroxisome of and assayed for production of IA on WCO. To further improve production yield, the 10 genes involved in the production pathway of acetyl-CoA, an intermediate metabolite necessary for the synthesis of cis-aconitic acid, were individually overexpressed and investigated for their impact on IA production. To minimize off-target flux channeling, we had also knocked out genes related to competing pathways in the peroxisome. Impressively, IA titer up to 54.55 g/L was achieved in our engineered in a 5 L bioreactor using WCO as the sole carbon source.

摘要

衣康酸（IA）是一种具有大量工业应用的高价值有机酸。在本研究中，我们旨在开发一种微生物细胞工厂，该工厂可以利用废食用油（WCO）作为原料，以循环且具有成本效益的方式生产上述生化物质。具体而言，我们将来源于[具体来源未提及]的顺乌头酸脱羧酶（CAD）基因在[具体微生物未提及]的细胞质或过氧化物酶体中进行表达，并检测其在WCO上生产衣康酸的情况。为了进一步提高产量，参与顺乌头酸合成所需的中间代谢产物乙酰辅酶A生产途径的10个基因被单独过表达，并研究它们对衣康酸生产的影响。为了尽量减少非靶向通量引导，我们还敲除了过氧化物酶体中与竞争途径相关的基因。令人印象深刻的是，在5升生物反应器中，我们构建的[具体微生物未提及]以WCO作为唯一碳源时，衣康酸产量达到了54.55克/升。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/cb1a0e6be89e/fbioe-10-888869-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/e2e675490af7/fbioe-10-888869-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/51a56a9ca3d8/fbioe-10-888869-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/7b9b451180d5/fbioe-10-888869-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/5cb889352c1e/fbioe-10-888869-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/7d5c410308bf/fbioe-10-888869-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/cb1a0e6be89e/fbioe-10-888869-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/e2e675490af7/fbioe-10-888869-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/51a56a9ca3d8/fbioe-10-888869-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/7b9b451180d5/fbioe-10-888869-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/5cb889352c1e/fbioe-10-888869-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/7d5c410308bf/fbioe-10-888869-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bd1/9083544/cb1a0e6be89e/fbioe-10-888869-g006.jpg

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