Photochemistry Department, National Research Centre, Giza, Dokki, Egypt.
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Biotechnol J. 2023 Oct;18(10):e2300093. doi: 10.1002/biot.202300093. Epub 2023 Jun 23.
In this study, novel crosslinked hydrogels based on chitosan (CS) and carrageenan (CRG) loaded with silver and/or copper nanoparticles (Ag/CuNPs) were prepared through a freeze-drying (thawing) process to be applied in biological applications comprising wound dressing. These hydrogels showed porous interconnected structures. The influence of the used nanoparticles (NPs) on the antibacterial properties of the CS/CRG hydrogels was explored. Antimicrobial results revealed that both CS/CRG/CuNPs, CS/CRG/AgNPs, and CS/CRG/Ag-CuNPs exhibited promising antibacterial and antifungal activity against Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, Staphylococcus aureus, Bacillus subtilis, and Candida albicans. Moreover, CS/CRG/AgNPs, CS/CRG/CuNPs, and CS/CRG/Ag-CuNPs hydrogels showed potential antioxidant activity to be 57%, 78%, and 89%, respectively. Furthermore, cytotoxicity results against Vero normal cell line confirmed that all designed hydrogels are safe upon usage. The bimetallic CS/CRG hydrogels showed notably enhanced antibacterial properties among the as-prepared hydrogels allowing them to be a successful material upon being employed in wound dressing applications.
在这项研究中，通过冷冻干燥（解冻）过程制备了基于壳聚糖（CS）和卡拉胶（CRG）的新型交联水凝胶，负载银和/或铜纳米粒子（Ag/CuNPs），用于包括伤口敷料在内的生物应用。这些水凝胶具有多孔互连结构。研究了所使用的纳米粒子（NPs）对 CS/CRG 水凝胶抗菌性能的影响。抗菌结果表明，CS/CRG/CuNPs、CS/CRG/AgNPs 和 CS/CRG/Ag-CuNPs 对大肠杆菌、铜绿假单胞菌、变形链球菌、金黄色葡萄球菌、枯草芽孢杆菌和白色念珠菌均表现出良好的抗菌和抗真菌活性。此外，CS/CRG/AgNPs、CS/CRG/CuNPs 和 CS/CRG/Ag-CuNPs 水凝胶的抗氧化活性分别为 57%、78%和 89%。此外，对 Vero 正常细胞系的细胞毒性结果证实，所有设计的水凝胶在使用时都是安全的。双金属 CS/CRG 水凝胶在制备的水凝胶中表现出显著增强的抗菌性能，使其成为伤口敷料应用中的一种成功材料。
