Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax, University of Sfax, P.O. 1173, 3038 Sfax, Tunisia.
Laboratory of Histology and Embryology, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.
Biochim Biophys Acta Gen Subj. 2019 Jan;1863(1):241-254. doi: 10.1016/j.bbagen.2018.10.010. Epub 2018 Oct 16.
The aim of the present study was to prepare chitosan-PVA-silver nanoparticles (CS-AgNPs) through green method. Chitosan and PVA polymers acted as stabilizing agents. DLS and TEM analyses showed that CS-AgNPs were homogeneously dispersed in matrix with an average size of 190-200 nm. The CS-AgNPs were tested for their antioxidant and antibacterial properties and the results revealed that they exhibited higher antioxidant activity than CS powder. Moreover, CS-AgNPs were characterized by a low cytotoxicity effect at 5-200 μg/ml against Chinese Hamster Ovary (CHO-K1) cells. In addition, the prepared CS-Ag NPs were found to promote significantly the wound healing, as determined by the wound contraction ratio and histological examination. A significant improvement in wound healing progression and in oxidative stress damage were observed for CS, CS-PVA and CS-AgNPs-treated wound tissues, when compared to control and CICAFLORA®-treated groups. The wound healing effect could be attributed to the antibacterial and antioxidant synergy of AgNPs and CS. Results strongly support the possibility of using CS-AgNPs for wound care applications.
本研究旨在通过绿色方法制备壳聚糖-聚乙烯醇-银纳米粒子（CS-AgNPs）。壳聚糖和聚乙烯醇聚合物充当稳定剂。DLS 和 TEM 分析表明，CS-AgNPs 均匀分散在基质中，平均粒径为 190-200nm。测试了 CS-AgNPs 的抗氧化和抗菌性能，结果表明其抗氧化活性高于 CS 粉末。此外，CS-AgNPs 在 5-200μg/ml 浓度下对中国仓鼠卵巢（CHO-K1）细胞的细胞毒性作用较低。此外，通过伤口收缩比和组织学检查，发现制备的 CS-Ag NPs 可显著促进伤口愈合。与对照组和 CICAFLORA®处理组相比，CS、CS-PVA 和 CS-AgNPs 处理的伤口组织中，伤口愈合过程和氧化应激损伤得到了显著改善。伤口愈合效果可能归因于 AgNPs 和 CS 的抗菌和抗氧化协同作用。结果强烈支持将 CS-AgNPs 用于伤口护理应用的可能性。
