Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, Queensland, Australia.
Centre for Clinical Research, Faculty of Medicine, The University of Queensland, Royal Brisbane and Women's Hospital, Brisbane, Queensland, Australia.
Nat Nanotechnol. 2023 Oct;18(10):1222-1229. doi: 10.1038/s41565-023-01415-1. Epub 2023 Jun 8.
Accurate and early detection of biomarkers provides the molecular evidence for disease management, allowing prompt actions and timely treatments to save lives. Multivalent biomolecular interactions between the probe and biomarker as well as controlled probe orientation on material surfaces are keys for highly sensitive detection. Here we report the bioengineering of programmable and multifunctional nanoprobes, which can provide rapid, specific and highly sensitive detection of emerging diseases in a range of widely used diagnostic systems. These nanoprobes composed of nanosized cell wall fragments, termed as synthetic bionanofragments (SynBioNFs), are generated by the fragmentation of genetically programmed yeast cells. SynBioNFs display multiple copies of biomolecules for high-affinity target binding and molecular handles for the precisely orientated attachment on surfaces used in diagnostic platforms. SynBioNFs are demonstrated for the capture and detection of SARS-CoV-2 virions using multiple diagnostic platforms, including surface-enhanced Raman scattering, fluorescence, electrochemical and colorimetric-based lateral flow systems with sensitivity comparable with the gold-standard reverse-transcription quantitative polymerase chain reaction.
准确和早期的生物标志物检测为疾病管理提供了分子证据，使人们能够及时采取行动并进行及时治疗，从而拯救生命。探针和生物标志物之间的多价生物分子相互作用以及探针在材料表面的受控取向是实现高灵敏度检测的关键。在这里，我们报告了可编程和多功能纳米探针的生物工程，这些探针可在一系列广泛使用的诊断系统中提供对新兴疾病的快速、特异和高灵敏度检测。这些由纳米级细胞壁片段组成的纳米探针，称为合成生物纳米片段（SynBioNFs），是通过遗传编程的酵母细胞的片段化产生的。SynBioNFs 显示出高亲和力靶标结合的多个生物分子拷贝和分子把手，用于在诊断平台上精确定向附着。SynBioNFs 被用于使用多种诊断平台（包括表面增强拉曼散射、荧光、电化学和比色横向流动系统）捕获和检测 SARS-CoV-2 病毒粒子，其灵敏度可与金标准逆转录定量聚合酶链反应相媲美。
