Institute of Marine Biology and Pharmacology, Ocean College, Zhejiang University, Zhoushan, 316021, P. R. China.
Zhejiang Institute for Food and Drug Control, Key Laboratory of Functional Food Nutrition and Quality Safety for State Market Regulation, Key Laboratory of Health Food Quality Safety of Provincial Market Regulation, Key Laboratory of Drug Contacting Materials Quality Control of Zhejiang Provincial, Hangzhou, 310052, P. R. China.
Chem Biodivers. 2023 Jul;20(7):e202300538. doi: 10.1002/cbdv.202300538. Epub 2023 Jun 28.
Alpiniamides E-G, three previously unreported linear polyketide derivatives, along with two known compounds, were isolated from Streptomyces sp. QHA48, which was isolated from the saline lakes of Qinghai-Tibet Plateau. The structures of these compounds were determined through analysis of their spectroscopic data, as well as density functional theory prediction of NMR chemical shifts, application of the DP4+ algorithm and electronic circular dichroism (ECD) calculations. In a cell-based lipid-lowering assay, all five alpiniamides exhibited significant inhibition of lipid accumulation in HepG2 cells without inducing cytotoxic effects at a concentration of 27 μM.
从青藏高原盐湖中分离到的链霉菌 QHA48 中分离得到了 Alpiniamides E-G，这是三种以前未报道的线性聚酮衍生物，以及两种已知化合物。通过分析它们的光谱数据以及通过密度泛函理论预测 NMR 化学位移、应用 DP4+算法和电子圆二色性（ECD）计算，确定了这些化合物的结构。在基于细胞的降脂测定中，所有 5 种 Alpiniamides 在 27 μM 的浓度下均表现出对 HepG2 细胞中脂质积累的显著抑制作用，而没有诱导细胞毒性作用。
