Department of Diagnosis and Surgery, Institute of Science and Technology, São Paulo State University, São José dos Campos, Brazil.
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, São José dos Campos, Brazil.
Am J Orthod Dentofacial Orthop. 2023 Aug;164(2):e43-e50. doi: 10.1016/j.ajodo.2023.05.014. Epub 2023 Jun 8.
This study aimed to assess the efficacy of chemical agents in removing Candida albicans and Streptococcus mutans biofilm from invisible aligners.
The samples were made of EX30 Invisalign trays, biofilm was cultured by standardized suspensions of C. albicans ATCC strain and S. mutans clinical strain on the sample. The treatments used were 0.5% sodium hypochlorite (NaClO) (20 minutes), 1% NaClO (10 minutes), chlorhexidine (5 minutes), peroxide (15 minutes), and orthophosphoric acid (15 seconds). The control group received phosphate-buffered saline for 10 minutes. The colony-forming units per milliliter of each microorganism were determined by serial dilutions seeded in plates with selective culture mediums for each one. Data were analyzed by the Kruskal-Wallis and Conover-Iman tests at an α of 0.05.
For the C. albicans biofilm group, the control group had 9.7 Log10 of microorganism growth, and all treatment groups had statistically significant biofilm reduction, in which chlorhexidine presented the highest inhibition of 3 Log10, followed by alkaline peroxide and orthophosphoric acid both with 2.6 Log10, 1% NaClO (2.5 Log10), and 0.5% NaClO (2 Log10). As for S. mutans, the control group had 8.9 Log10 of growth, and a total microorganism inhibition was reached by chlorhexidine, 1% NaClO, and orthophosphoric acid, whereas alkaline peroxide inhibited growth to 7.9 Log10 and 0.5% NaClO 5.1 Log10.
Within the limitations, chlorhexidine and orthophosphoric acid had greater efficacy in both biofilms. In addition, 1% NaClO and alkaline peroxide also had significant effects; therefore, their incorporation aligners disinfection protocols are valid.
本研究旨在评估化学制剂清除隐形矫正器中白色念珠菌和变异链球菌生物膜的效果。
样本由 EX30 隐适美托盘制成，通过将白色念珠菌 ATCC 株和变异链球菌临床株的标准悬浮液培养在样本上形成生物膜。使用的处理方法为 0.5%次氯酸钠（NaClO）（20 分钟）、1%NaClO（10 分钟）、洗必泰（5 分钟）、过氧化物（15 分钟）和正磷酸（15 秒）。对照组用磷酸盐缓冲盐水处理 10 分钟。通过在含有每种微生物选择性培养基的平板上进行系列稀释来确定每毫升微生物的菌落形成单位数。数据采用 Kruskal-Wallis 和 Conover-Iman 检验进行分析，α 值为 0.05。
对于白色念珠菌生物膜组，对照组的微生物生长为 9.7 Log10，所有处理组的生物膜减少均具有统计学意义，其中洗必泰的抑制效果最高，达到 3 Log10，其次是碱性过氧化物和正磷酸，均为 2.6 Log10，1%NaClO（2.5 Log10）和 0.5%NaClO（2 Log10）。对于变异链球菌，对照组的生长为 8.9 Log10，洗必泰、1%NaClO 和正磷酸达到了对所有微生物的抑制作用，而碱性过氧化物抑制生长至 7.9 Log10，0.5%NaClO 抑制生长至 5.1 Log10。
在限制条件下，洗必泰和正磷酸对两种生物膜均具有更强的功效。此外，1%NaClO 和碱性过氧化物也具有显著效果；因此，它们在隐形矫正器消毒方案中的应用是有效的。
