Jamia Hamdard, Centre of Excellence in Unani Medicine (Pharmacognosy & Pharmacology), & Bioactive Natural Product Laboratory, School of Pharmaceutical Education & Research, New Delhi 110062, India.
Dabur India Limited,Dabur Research and Development Centre, Plot No. 22, Site IV, Sahibabad, Ghaziabad, Uttar Pradesh 201010, India.
J AOAC Int. 2023 Sep 1;106(5):1305-1312. doi: 10.1093/jaoacint/qsad070.
Due to its medicinal properties, Pistacia integerrima is in high demand and is extensively used as a key ingredient in various formulations. However, its popularity has led to its inclusion on the International Union for Conservation of Nature threatened category list. In Ayurvedic texts, such as Bhaishajaya Ratnavali, Quercus infectoria is recommended as a substitute for P. integerrima in different formulations. Additionally, Yogratnakar highlights that Terminalia chebula shares similar therapeutic properties with P. integerrima.
The objective of the current study was to gather scientific data on metabolite profiling and marker-based comparative analysis of Q. infectoria, T. chebula, and P. integerrima.
In present study, hydroalcoholic and aqueous extracts of all three plants were prepared and standardized for the comparative evaluation of secondary metabolites. TLC was carried out for the comparative fingerprinting of the extracts using chloroform-methanol-glacial acetic acid-water (60 + 8 + 32 + 10, by volume) as a solvent system. A fast, sensitive, selective, and robust HPLC method was developed to determine gallic acid and ellagic acid from both extracts of all three plants. The method was validated for precision, robustness, accuracy, LOD and LOQ as per the International Conference on Harmonization guidelines.
The TLC analysis revealed the presence of several metabolites, and the pattern of metabolites in the plants exhibited a certain degree of similarity. A highly precise and reliable quantification technique was created for gallic acid and ellagic acid, operating within a linear concentration range of 81.18-288.22 µg/mL and 3.83-13.66 µg/mL, respectively. The correlation coefficients for gallic acid and ellagic acid were 0.997 and 0.996, indicating good linear relationships. The gallic acid content in all three plants ranged from 3.74 to 10.16% w/w, while the ellagic acid content ranged from 0.10 to 1.24% w/w.
The study contributes to the scientific understanding of the metabolite profiles and comparative analysis of Q. infectoria, T. chebula, and P. integerrima. The findings provide valuable insights into the chemical composition of these plants and can be used for various applications in herbal medicine.
This pioneering scientific approach highlights the phytochemical similarities between Q. infectoria, T. chebula and P. integerrima.
由于其药用特性，野骆驼蓬被广泛需求，并被广泛用作各种配方的关键成分。然而，它的受欢迎程度导致它被列入国际自然保护联盟濒危物种名单。在《阿育吠陀本草纲目》等阿育吠陀文献中，如《八色甘露》，推荐在不同配方中用栓皮栎替代野骆驼蓬。此外，《药论》强调诃子与野骆驼蓬具有相似的治疗特性。
本研究的目的是收集关于栓皮栎、诃子和野骆驼蓬的代谢物特征图谱和基于标志物的比较分析的科学数据。
本研究中，制备了三种植物的水醇和水提物，并对其进行了标准化，以进行次生代谢物的比较评价。采用氯仿-甲醇-冰醋酸-水（60+8+32+10，体积比）作为溶剂系统，进行 TLC 比较指纹图谱分析。建立了一种快速、灵敏、选择性和稳健的 HPLC 方法，用于测定三种植物提取物中均有的没食子酸和鞣花酸。该方法根据国际协调会议指南进行了精密度、稳健性、准确性、LOD 和 LOQ 的验证。
TLC 分析显示存在多种代谢物，且植物中的代谢物模式表现出一定程度的相似性。建立了一种用于没食子酸和鞣花酸的高度精确和可靠的定量技术，操作范围分别为 81.18-288.22μg/mL 和 3.83-13.66μg/mL。没食子酸和鞣花酸的相关系数分别为 0.997 和 0.996，表明具有良好的线性关系。三种植物中的没食子酸含量范围为 3.74%-10.16%w/w，鞣花酸含量范围为 0.10%-1.24%w/w。
本研究有助于对栓皮栎、诃子和野骆驼蓬的代谢物特征图谱和比较分析进行科学理解。研究结果为这些植物的化学成分提供了有价值的见解，并可用于草药医学中的各种应用。
这种开创性的科学方法突出了栓皮栎、诃子和野骆驼蓬之间的植物化学相似性。
