[异硫氰酸荧光素标记的干扰素-α对脑肿瘤的抗肿瘤作用及作用机制]

[Antineoplastic effects and mechanism of action of IFN-alpha on brain tumors with use of fluorescent isothiocyanate-labelled IFN].

作者信息

Kawamoto K, Numa Y, Fujiwara H, Matsumura H, Ikegami H, Kurimoto M, Toriyama K

出版信息

Gan To Kagaku Ryoho. 1986 Jul;13(7):2346-52.

Abstract

The antineoplastic effects and mechanism of IFN-alpha with a newly designed fluorescent isothiocyanate-labelled IFN-alpha were studied on three established glioma-cell lines and cultured cells of clinical brain tumors. Viability, cell cycle and cells showing positive reaction with fluorescent isothiocyanate-labelled IFN were analyzed by flow cytometry. U373MG glioma cells in contact with 10(4) IU/ml of IFN for 24 hours had a dose dependency, decreased viability, S-phase block in the cell cycle and high rate of positive reaction with fluorescent isothiocyanate-labelled IFN. Two surgical cases, except for one case, showed similar results and other cell lines or meningiomas were not affected by IFN. As the antineoplastic effects of IFN-alpha correlated with the rate of occurrence of positive cells with fluorescent isothiocyanate-labelled IFN, sensitive tumor cells may have a respective specific receptor for IFN-alpha, and, therefore, fluorescent isothiocyanate-labelled IFN-alpha might be of clinical value with regard to the problem of the receptor as well as sensitivity test.

摘要

利用新设计的异硫氰酸荧光素标记的α-干扰素（IFN-α），对三种已建立的胶质瘤细胞系和临床脑肿瘤培养细胞进行了抗肿瘤作用及机制的研究。通过流式细胞术分析细胞活力、细胞周期以及对异硫氰酸荧光素标记的IFN呈阳性反应的细胞。与10⁴IU/ml的IFN接触24小时的U373MG胶质瘤细胞具有剂量依赖性，细胞活力降低，细胞周期出现S期阻滞，且对异硫氰酸荧光素标记的IFN呈阳性反应的比例较高。两例手术病例，除一例之外，均显示出相似结果，而其他细胞系或脑膜瘤未受IFN影响。由于IFN-α的抗肿瘤作用与异硫氰酸荧光素标记的IFN阳性细胞的出现率相关，敏感肿瘤细胞可能具有各自特定的IFN-α受体，因此，异硫氰酸荧光素标记的IFN-α在受体问题以及敏感性测试方面可能具有临床价值。