Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China.
Clinical Molecular Medicine Testing Center, The First Affiliated Hospital of Chongqing Medical University, College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China.
Biosens Bioelectron. 2023 Oct 1;237:115407. doi: 10.1016/j.bios.2023.115407. Epub 2023 May 27.
Electrochemical nucleic acid biosensor has demonstrated great promise in clinical diagnostic tests, mainly because of its flexibility, high efficiency, low cost, and easy integration for analytical applications. Numerous nucleic acid hybridization-based strategies have been developed for the design and construction of novel electrochemical biosensors for diagnosing genetic-related diseases. This review describes the advances, challenges, and prospects of electrochemical nucleic acid biosensors for mobile molecular diagnosis. Specifically, the basic principles, sensing elements, applications in diagnosis of cancer and infectious diseases, integration with microfluidic technology and commercialization are mainly included in this review, aiming to provide new insights and directions for the future development of electrochemical nucleic acid biosensors.
电化学核酸生物传感器在临床诊断测试中表现出巨大的潜力，主要是因为它具有灵活性、高效率、低成本和易于集成分析应用等优点。已经开发了许多基于核酸杂交的策略，用于设计和构建用于诊断与遗传相关疾病的新型电化学生物传感器。本综述描述了用于移动分子诊断的电化学核酸生物传感器的进展、挑战和前景。具体来说，本综述主要包括电化学核酸生物传感器的基本原理、传感元件、在癌症和传染病诊断中的应用、与微流控技术的集成以及商业化等方面，旨在为电化学核酸生物传感器的未来发展提供新的见解和方向。
