School of Materials Science and Engineering, Nanjing University of Science & Technology, Nanjing 210094, PR China; Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, PR China.
Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, PR China; Cixi Biomedical Research Institute, Wenzhou Medical University, Zhejiang 325035, PR China.
Int J Biol Macromol. 2023 Jul 15;243:125249. doi: 10.1016/j.ijbiomac.2023.125249. Epub 2023 Jun 7.
Trauma-induced articular cartilage damages are common in clinical practice. Hydrogels have been used to fill the cartilage defects and act as extracellular matrices for cell migration and tissue regeneration. Lubrication and stability of the filler materials are essential to achieve a satisfying healing effect in cartilage regeneration. However, conventional hydrogels failed to provide a lubricous effect, or could not anchor to the wound to maintain a stable curing effect. Herein, we fabricated dually cross-linked hydrogels using oxidized hyaluronic acid (OHA) and N-(2-hydroxypropyl)-3-trimethylammonium chitosan chloride (HTCC) methacrylate (HTCCMA). The OHA/HTCCMA hydrogels, which were dynamically cross-linked and then covalently cross-linked by photo-irradiation, showed appropriate rheological properties and self-healing capability. The hydrogels exhibited moderate and stable tissue adhesion property due to formation of dynamic covalent bonds with the cartilage surface. The coefficient of friction values were 0.065 and 0.078 for the dynamically cross-linked and double-cross-linked hydrogels, respectively, demonstrating superior lubrication. In vitro studies showed that the hydrogels had good antibacterial ability and promoted cell proliferation. In vivo studies confirmed that the hydrogels were biocompatible and biodegradable, and exhibited a robust regenerating ability for articular cartilage. This lubricant-adhesive hydrogel is expected to be promising for the treatment of joint injuries as well as regeneration.
创伤性关节软骨损伤在临床实践中很常见。水凝胶已被用于填充软骨缺陷，并作为细胞迁移和组织再生的细胞外基质。填充材料的润滑和稳定性对于实现软骨再生的令人满意的愈合效果至关重要。然而，传统的水凝胶无法提供润滑效果，或者无法固定在伤口处以保持稳定的固化效果。在此，我们使用氧化透明质酸（OHA）和 N-(2-羟丙基)-3-三甲基氯化铵壳聚糖盐酸盐（HTCC）甲基丙烯酰胺（HTCCMA）制备了双重交联水凝胶。OHA/HTCCMA 水凝胶通过光辐照进行动态交联，然后进行共价交联，具有适当的流变性能和自修复能力。由于与软骨表面形成动态共价键，水凝胶具有适中且稳定的组织粘附性能。动态交联和双交联水凝胶的摩擦系数值分别为 0.065 和 0.078，表明具有优异的润滑性。体外研究表明，水凝胶具有良好的抗菌能力并促进细胞增殖。体内研究证实，水凝胶具有良好的生物相容性和可生物降解性，并对关节软骨具有强大的再生能力。这种具有润滑和粘附性能的水凝胶有望成为治疗关节损伤和再生的有前途的方法。
