Department of Molecular Plant Physiology, Institute of Environmental Biology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02096 Warsaw, Poland.
Cells. 2023 May 26;12(11):1480. doi: 10.3390/cells12111480.
The aim of this study was to examine how light intensity and quality affect the photosynthetic apparatus of cells by modulating the structure and function of phycobilisomes. Cells were grown in equal amounts of white, blue, red, and yellow light of low (LL) and high (HL) intensity. Biochemical characterization, fluorescence emission, and oxygen exchange were used to investigate selected cellular physiological parameters. It was found that the allophycocyanin content was sensitive only to light intensity, whereas the phycocynin content was also sensitive to light quality. Furthermore, the concentration of the PSI core protein was not affected by the intensity or quality of the growth light, but the concentration of the PSII core D1 protein was. Finally, the amount of ATP and ADP was lower in HL than LL. In our opinion, both light intensity and quality are main factors that play an important regulatory role in acclimatization/adaptation of to environmental changes, and this is achieved by balancing the amounts of thylakoid membrane and phycobilisome proteins, the energy level, and the photosynthetic and respiratory activity. This understanding contributes to the development of a mix of cultivation techniques and genetic changes for a future large-scale synthesis of desirable biomolecules.
本研究旨在通过调节藻胆体的结构和功能来研究光强和光质如何影响细胞的光合作用器官。将细胞在低（LL）和高光强（HL）的白光、蓝光、红光和黄光下等比例生长。使用生化特性分析、荧光发射和氧气交换来研究选定的细胞生理参数。结果发现，别藻蓝蛋白含量仅对光强敏感，而藻红蛋白含量也对光质敏感。此外，PSI 核心蛋白的浓度不受生长光强或质量的影响，但 PSII 核心 D1 蛋白的浓度受影响。最后，HL 中的 ATP 和 ADP 含量低于 LL。我们认为，光强和光质都是藻类适应环境变化的重要调节因素，这是通过平衡类囊体膜和藻胆体蛋白的数量、能量水平以及光合和呼吸活性来实现的。这种理解有助于开发混合培养技术和遗传改变，以实现未来大规模合成所需生物分子的目标。
