Bratei Alexandru Adrian, Stefan-van Staden Raluca-Ioana
Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, 011061 Bucharest, Romania.
Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 060021 Bucharest, Romania.
Diagnostics (Basel). 2023 May 26;13(11):1857. doi: 10.3390/diagnostics13111857.
(1) Background: Human SERPINB5, commonly known as maspin, has diverse functions as a tumor suppressor. Maspin has a novel role in cell cycle control, and common variants were discovered to be associated with gastric cancer (GC). Maspin was proven to also affect the EMT and angiogenesis of gastric cancer cells via the ITGB1/FAK pathway. Information about the maspin concentrations correlated with different pathological features of the patients may facilitate the fast diagnosis and personalized treatment of patients. The novelty of this study is given by the correlations established for the maspin levels in different biological features and clinicopathological features. These correlations can be extremely useful for surgeons and oncologists. (2) Patients and methods: Patients with clinical and pathological features, given the small number of samples available for this study, were selected from the database of the project GRAPHSENSGASTROINTES, and used in accordance with the Ethics Committee approval nr. 32,647/2018 awarded by the County Emergency Hospital from Targu-Mures. Stochastic microsensors were used as new screening tools for the determination of the concentration of maspin in four types of samples: tumoral tissues, blood, saliva and urine. (3) Results: The results obtained using the stochastic sensors were correlated with those tabulated in the clinical and pathological database. A series of assumptions regarding the values and practice important features for surgeons and pathologists were made. (4) Conclusions: This study provided a few assumptions regarding the correlations between the values of maspin levels in the analyzed samples and the clinical and pathological features. These results may be useful as preoperative investigations in order to help surgeons localize, approximate and choose the best treatment. These correlations may facilitate minim invasive and fast diagnosis of gastric cancer based on reliable detection of maspin concentration in biological samples (tumoral tissues, blood, saliva and urine).
(1) 背景：人类丝氨酸蛋白酶抑制剂B5（SERPINB5），通常称为乳腺丝抑蛋白（maspin），作为一种肿瘤抑制因子具有多种功能。Maspin在细胞周期控制中具有新作用，并且发现常见变异与胃癌（GC）相关。已证实Maspin还通过整合素β1（ITGB1）/黏着斑激酶（FAK）途径影响胃癌细胞的上皮-间质转化（EMT）和血管生成。关于与患者不同病理特征相关的maspin浓度的信息可能有助于患者的快速诊断和个性化治疗。本研究的新颖之处在于建立了不同生物学特征和临床病理特征中maspin水平的相关性。这些相关性对外科医生和肿瘤学家可能极其有用。(2) 患者与方法：鉴于本研究可用样本数量较少，具有临床和病理特征的患者从GRAPHSENSGASTROINTES项目数据库中选取，并根据特尔古穆列什县急救医院授予的第32,647/2018号伦理委员会批准意见使用。随机微传感器用作新的筛查工具，用于测定四种类型样本（肿瘤组织、血液、唾液和尿液）中maspin的浓度。(3) 结果：使用随机传感器获得的结果与临床和病理数据库中列出的结果相关。针对外科医生和病理学家的数值及实践重要特征提出了一系列假设。(4) 结论：本研究提供了一些关于分析样本中maspin水平值与临床和病理特征之间相关性的假设。这些结果作为术前检查可能有用，以帮助外科医生定位、评估并选择最佳治疗方案。基于对生物样本（肿瘤组织、血液、唾液和尿液）中maspin浓度的可靠检测，这些相关性可能有助于胃癌的微创和快速诊断。
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