Department of Physical Chemistry, Faculty of Pharmacy, Medical University of Lublin, 4a Chodźki Str., 20-093 Lublin, Poland.
Int J Mol Sci. 2023 May 28;24(11):9389. doi: 10.3390/ijms24119389.
High-performance layer electrochromatography (HPLEC) combines the advantages of overpressured-layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC) while overcoming some of their limitations. HPLEC equipment can work in various HPLEC, OPLC, and PPEC modes. The equipment enables HPLEC analysis also with an electroosmotic effect directed against the hydrodynamic flow of the mobile phase. The change in the electric field direction in the separation system does not result in a change in either the direction of the mobile phase flow or the direction of solute migration. The hydrodynamic flow generated by the pump dominates the electroosmotic effect and enables separation against the direction of the latter. Reversed-polarization HPLEC may be advantageous for the analysis of anionic compounds, as it facilitates faster and more selective separation than OPLC performed in similar conditions. This separation mode provides a new possibility to develop and optimize separation methods by performing separation against the electroosmotic effect and without need of any modification of the adsorbent surface. A drawback of this separation mode is the increase in the backpressure at the mobile phase inlet and the limitation of the mobile phase flow rate. Currently, contrary to the single-channel mode, multi-channel reverse-polarity HPLEC still requires some technical and methodological improvements.
高效层电色谱（HPLEC）结合了高压层色谱（OPLC）和加压平面电色谱（PPEC）的优点，同时克服了它们的一些局限性。HPLEC 设备可以在各种 HPLEC、OPLC 和 PPEC 模式下工作。该设备还可以在电渗流方向与流动相的流体力学流动相反的情况下进行 HPLEC 分析。分离系统中电场方向的变化不会导致流动相流动方向或溶质迁移方向的变化。泵产生的流体力学流动主导着电渗流效应，并能够与后者的方向相反进行分离。反相 HPLEC 可能有利于阴离子化合物的分析，因为与在类似条件下进行的 OPLC 相比，它可以实现更快、更选择性的分离。这种分离模式通过对电渗流效应进行分离而无需对吸附剂表面进行任何修饰，为开发和优化分离方法提供了新的可能性。这种分离模式的缺点是流动相入口处的背压增加和流动相流速的限制。目前，与单通道模式相反，多通道反向极性 HPLEC 仍需要一些技术和方法上的改进。
