Department of Physical Chemistry, Chair of Chemistry, Faculty of Pharmacy, Medical University of Lublin, 4a Chodźki Str., 20-093 Lublin, Poland.
Anal Chem. 2022 Jun 28;94(25):9091-9096. doi: 10.1021/acs.analchem.2c01376. Epub 2022 Jun 14.
We present a new prototype device and propose a new analytical technique: high performance (high pressure) layer electrochromatography (HPLEC). The equipment provides a combination of overpressured layer chromatography (OPLC) and pressurized planar electrochromatography (PPEC), yet it still enables researchers to perform each of these analyses separately. In comparison to PPEC, HPLEC provides hydrodynamic flow of the mobile phase, irrespective of the voltage used and the mobile phase composition. The advantages of HPLEC over OPLC include the possibility of the use of the electrophoretic effect to influence the selectivity of separation and the use of the electroosmotic effect to facilitate the mobile phase flow in order to decrease backpressure and increase the flow velocity. Many operational parameters can be freely adjusted and optimized independently. The equipment is fully automated and can work in various separation/operational modes, including combinations of online/offline sample application and detection. We present preliminary results of simultaneous, fully online, multichannel HPLEC separation of analgesic drugs (including acetaminophen, ibuprofen, and tramadol) as an example of increasing analysis throughput.
高性能（高压）层电色层析（HPLEC）。该设备结合了超压层色层析（OPLC）和加压平面电色层析（PPEC），但仍允许研究人员分别进行这两种分析。与 PPEC 相比，HPLEC 提供了流动相的流体动力学流动，而与所使用的电压和流动相组成无关。HPLEC 相对于 OPLC 的优势包括使用电泳效应影响分离选择性的可能性，以及使用电渗效应来促进流动相流动以降低背压并增加流速的可能性。许多操作参数可以自由调整和优化。该设备完全自动化，可以在各种分离/操作模式下工作，包括在线/离线样品应用和检测的组合。我们以同时进行的、完全在线的、多通道 HPLEC 分离镇痛药物（包括对乙酰氨基酚、布洛芬和曲马多）为例，展示了提高分析通量的初步结果。
