Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
J Chromatogr A. 2011 May 13;1218(19):2636-47. doi: 10.1016/j.chroma.2011.03.014. Epub 2011 Mar 17.
Theoretical backgrounds, development, examples of separations, constructional details and principle of action of devices of pressurized planar electrochromatography (PPEC) are presented. Development of the mode is described in respect of operating variables (composition of the mobile phase, pressure exerted on adsorbent layer, mobile phase flow velocity, temperature of separating system, etc.) influencing separation efficiency (kinetic performance, repeatability, separation time). Advantages of PPEC such as high kinetic performance, short separation time and different separation selectivities, especially relative to conventional thin-layer chromatography, are described. Examples of two-dimensional separations are demonstrated to show high separation potential of the mode when combined with conventional thin-layer chromatography (TLC). The PPEC mode is in infancy stage of development, so its challenges are presented as well.
介绍了加压平面电色层析（PPEC）设备的理论背景、发展、分离实例、结构细节和作用原理。从影响分离效率（动力学性能、重现性、分离时间）的操作变量（流动相组成、吸附剂层所受压力、流动相流速、分离系统温度等）方面描述了该模式的发展。描述了 PPEC 的优点，例如高动力学性能、短分离时间和不同的分离选择性，特别是相对于传统的薄层色谱法。展示了二维分离的实例，以表明当与传统的薄层色谱法（TLC）结合时，该模式具有很高的分离潜力。PPEC 模式仍处于发展的初级阶段，因此也提出了其面临的挑战。
