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预处理绒毛膜间质干细胞（CVMSCs）可减少体外乳腺癌细胞系 MDA231 的侵袭表型。

Preconditioned Chorionic Villus Mesenchymal Stem/Stromal Cells (CVMSCs) Minimize the Invasive Phenotypes of Breast Cancer Cell Line MDA231 In Vitro.

机构信息

Blood and Cancer Research Department, King Abdullah International Medical Research Center (KAIMRC), King Saud Bin Abdulaziz University for Health Sciences (KSAU), Ministry of National Guard Health Affairs (MNGHA), Riyadh 11426, Saudi Arabia.

Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

出版信息

Int J Mol Sci. 2023 May 31;24(11):9569. doi: 10.3390/ijms24119569.

DOI:10.3390/ijms24119569
PMID:37298519
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10253955/
Abstract

Among the newer choices of targeted therapies against cancer, stem cell therapy is gaining importance because of their antitumor properties. Stem cells suppress growth, metastasis, and angiogenesis, and induce apoptosis in cancer cells. In this study, we have examined the impact of the cellular component and the secretome of preconditioned and naïve placenta-derived Chorionic Villus Mesenchymal Stem Cells (CVMSCs) on the functional characteristics of the Human Breast Cancer cell line MDA231. MDA231 cells were treated with preconditioned CVMSCs and their conditioned media (CM), followed by an evaluation of their functional activities and modulation in gene and protein expression. Human Mammary Epithelial Cells (HMECs) were used as a control. CM obtained from the preconditioned CVMSCs significantly altered the proliferation of MDA231 cells, yet no change in other phenotypes, such as adhesion, migration, and invasion, were observed at various concentrations and time points tested. However, the cellular component of preconditioned CVMSCs significantly inhibited several phenotypes of MDA231 cells, including proliferation, migration, and invasion. CVMSCs-treated MDA231 cells exhibited modulation in the expression of various genes involved in apoptosis, oncogenesis, and Epithelial to Mesenchymal Transition (EMT), explaining the changes in the invasive behavior of MDA231 cells. These studies reveal that preconditioned CVMSCs may make useful candidate in a stem cell-based therapy against cancer.

摘要

在针对癌症的新型靶向治疗方法中，干细胞疗法因其抗肿瘤特性而变得越来越重要。干细胞抑制肿瘤的生长、转移和血管生成，并诱导癌细胞凋亡。在这项研究中，我们研究了预处理和未预处理胎盘来源绒毛膜间质干细胞（CVMSCs）的细胞成分和分泌组对人乳腺癌细胞系 MDA231 的功能特征的影响。用预处理的 CVMSCs 及其条件培养基（CM）处理 MDA231 细胞，然后评估它们的功能活性和基因及蛋白表达的调节。用人乳腺上皮细胞（HMECs）作为对照。从预处理的 CVMSCs 获得的 CM 显著改变了 MDA231 细胞的增殖，但在不同浓度和时间点测试时，对其他表型（如粘附、迁移和侵袭）没有观察到变化。然而，预处理的 CVMSCs 的细胞成分显著抑制了 MDA231 细胞的几种表型，包括增殖、迁移和侵袭。用 CVMSCs 处理的 MDA231 细胞表现出参与细胞凋亡、致癌和上皮间质转化（EMT）的各种基因表达的调节，解释了 MDA231 细胞侵袭行为的变化。这些研究表明，预处理的 CVMSCs 可能成为基于干细胞的癌症治疗的有用候选物。

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